【作者】 王朴；

【导师】 任建生；

【作者基本信息】 武汉体育学院 ， 运动人体科学， 2007， 硕士

【摘要】 目的:通过茯苓煎剂联合有氧运动对高脂血症大鼠的干预,观察高脂血症大鼠血清TG、TC、HDL-C、LDL-C的浓度变化,以及黏附分子sICAM-1的浓度变化,以期进一步探讨高脂血症致动脉粥样硬化的形成机制和预防效果。方法:运用酶法测定大鼠血清中TG、TC、HDL-C、LDL-C含量,采用酶联免疫吸附法(ELISA)法测定血清中sICAM-1浓度。结果:与空白组相比:高脂模型组肥胖判别指数(Lee Index)显著升高(P<0.05),血清中TG、TC、LDL-C显著升高(P<0.05),HDL-C含量降低不显著(P>0.05),血清中sICAM-1显著增高(P<0.05)。与高脂模型组比较:①单纯茯苓煎剂使高脂饮食状态下形成的高脂血症大鼠的肥胖判别指数(Lee Index)显著降低(P<0.05)、血清中TG、TC、LDL-C含量显著降低(P<0.05)、HDL-C显著升高(P<0.05),血清中sICAM-1显著降低(P<0.05)。②茯苓煎剂联合有氧运动则显著降低肥胖判别指数(Lee Index)(P<0.05),血清中TG、TC、LDL-C含量显著降低(P<0.05)且显著升高HDL-C含量(P<0.05),血清中sICAM-1显著降低(P<0.05)。与单纯茯苓煎剂灌药组比较:茯苓煎剂联合有氧运动显著降低肥胖判别指数(Lee Index)(P<0.05)、显著降低血清中LDL-C含量(P<0.05),显著升高HDL-C含量(P<0.05),显著降低血清中sICAM-1含量(P<0.05),而TC、TG降低不显著(P>0.05)。结论:对于高脂饮食而形成高脂血症的大鼠,中药茯苓有一定的治疗作用,但以有氧运动联合茯苓煎剂的作用效果最好。灌药组大鼠的TG、TC、LDL-C和sICAM-1水平显著低于高脂模型组,而HDL-C水平显著高于高脂模型组。茯苓煎剂联合有氧运动组大鼠的TG、TC、LDL-C和sICAM-1水平显著低于高脂模型组,HDL-C显著升高。单纯茯苓煎剂和茯苓煎剂联和有氧运动均可以下调主动脉壁黏附分子的表达,提示对高脂血症的治疗应采取多因素协同的方法增强疗效。更多还原

【Abstract】 Destination:With the fuling combining aerobic training to act on hyperlipidemia, we research the concentration change of TG、TC、HDL-C、LDL-C in the blood serum of the hyperlipidemia rats, include the concentration change of adhesive molecule sICAM-1, in order to probe more into the causation and prevention of the atherosclerosis which is caused by hyperlipidemia.Technique:We measured the content of TG、TC、HDL-C、LDL-C by enzyme method, and the content of the sICAM-1 by ELISA.Results:Compared with the blank group:Fat Lee Index in high fat group increased distinctively(P<0.05), TG、TC、LDL-C increased distinctively(P<0.05), HDL-C reduced indistinctively(P<0.05), sICAM-1 increased distinctively(P<0.05).Compared with the high fat feedstuff group:①Fat Lee Index in fuling used only group reduced distinctively(P<0.05), TG、TC、LDL-C reduced distinctively(P<0.05), HDL-C increased distinctively(P<0.05), sICAM-1 reduced distinctively(P<0.05).②Fat Lee Index in fuling combining aerobic training group reduced distinctively(P<0.05), TG、TC、LDL-C reduced distinctively(P<0.05), HDL-C increased distinctively(P<0.05), sICAM-1 reduced distinctively(P<0.05).Compared with the fuling used only group: Fat Lee Index in fuling combining aerobic reduced distinctively(P<0.05), LDL-C reduced distinctively(P<0.05), HDL-C increased distinctively(P<0.05), sICAM-1 reduced distinctively(P<0.05), but the reduce of TC、TG is indistinctive(P<0.05).Conclusions:Chinese traditional medicine fuling has some remedial function to atherosclerosis rats which are caused by hyperlipidemia. While the best way is using the fuling combining aerobic training. The TG、TC and LDL-C levels of the group that is used fuling only are lower distinctively than that of group which is used high-fat feedstuff. While the level of HDL-C is higher distinctively than that. The TG、TC and LDL-C levels of the group that is used fuling combing aerobic training are lower distinctively than that of group which is used high-fat feedstuff, and the HDL-C increase distinctively. Fuling combining aerobic training can reduce the expression of the adhesive molecule in aortic paries. It wise up us that we can take many measures together to treat hyperlipidemia.更多还原