【作者】 刘强；

【导师】 王德江；

【作者基本信息】 山东大学 ， 胸外科， 2007， 硕士

【摘要】 目的检测转录因子E2F-1在原发性肺癌组织中的表达水平，并探讨其临床意义。方法本研究应用免疫组化S-P法，对64例原发性肺癌组织中转录因子E2F-1的表达进行检测，并对临床资料与实验结果之间的关系进行了分析。结果①64例原发性肺癌中，转录因子E2F-1表达阳性率为76.6％(49／64)，癌旁正常肺组织中转录因子E2F-1表达阳性率为10.9％(7／64)，表达阳性率显著高于癌旁正常肺组织组，统计学分析两组比较有显著性差异(P＜0.01，x~2=53.4)；②Ⅰ期原发性肺癌中，转录因子E2F-1蛋白阳性表达率为75.0％(9／12)，Ⅱ期为73.1％(19／26)，Ⅲ期为78.3％(18／23)，Ⅳ期为100％(3／3)，统计学分析四组间比较无显著性差异(P＞0.05，x~2=1.008)；③鳞状细胞癌中，转录因子E2F-1蛋白阳性表达率为58.3％(14／24)，腺癌为82.6％(19／23)，小细胞肺癌为92.3％(12／13)，大细胞肺癌为100％(4／4)，统计学分析四组间比较有显著性差异(P＜0.05，x~2=7.936)；④高分化型肺癌中，转录因子E2F-1蛋白阳性表达率52.9％(9／17)，中分化型76.0％(19／25)，低分化型95.5％(21／22)，统计学分析，三组间比较有显著性差异(P<0.01，x~2=9.664)；⑤有淋巴结转移组，转录因子E2F-1蛋白阳性表达率为79.5％(31／39)，无淋巴结转移组为72.0％(18／25)，统计学分析两组比较无显著性差异(P>0.05，x~2=0.150)；⑥未侵及脏胸膜组，转录因子E2F-1蛋白阳性表达率为78.6％(22／28)，侵及脏胸膜或周围临近组织组为75.0％(27／36)，统计学分析两组比较无显著性差异(P＞0.05，x~2=0.001)。结论1．采用免疫组化染色检测原发性肺癌组织中转录因子E2F-1的表达，发现转录因子E2F-1的表达高于正常肺组织。E2F-1蛋白的超表达可能是原发性肺癌发生发展的重要因素之一。2．免疫组化检测显示，转录因子E2F-1的表达与原发性肺癌的病理类型和分化程度有关：小细胞肺癌和大细胞肺癌中，E2F-1蛋白表达最高，腺癌次之，鳞癌最低；分化程度越低，E2F-1蛋白的表达就越高；但其表达与临床分期、淋巴结转移和肿瘤是否有局部浸润无关。3．综合分析转录因子E2F-1的表达与临床病理之间的关系，对原发性肺癌的基因诊断、基因治疗、选择合理的治疗方案和判断预后有重要参考价值。4．总之，原发性肺癌中转录因子E2F-1超表达和反常表达与原发性肺癌的发生发展有关，在肿瘤的发生发展中扮演者重要的角色。更多还原

【Abstract】 Objective To investigate the expression level and their clinical significance of transcription factor E2F-1 in primary lung carcinoma.Methods Using S-P immunohistochemistry, the expression of transcription factor E2F-1 was investigated in 64 primary lung carcinoma tissue samples. Statistical analysis was done to analyze the relationship between the clinical data and the experimental results.Results (1)The positive expression rate of transcription factor E2F-1 in 64 primary lung carcinoma tissues (76.0%) was markedly higher than that in normal lung tissues(10.9%)( P<0.01). ②The positive rate of transcription factor E2F-1 in stage I was 75.0%, that in stage II was 73.1%, in stage III was 78.3%, and in stage IVwas100%, there were no association in the clinical stage. (3)The positive rate of transcription factor E2F-1 in squamous carcinoma was 58.3%, which in adenocarcinoma was 82.6%, in small cell lung carcinoma was 92.3%, and in large cell lung carcinoma was 100%, which were significantly different. ④The positive expression rate of E2F-1 in high, middle and low differentiated lung carcinoma were respectively 52.9%, 76.0%, 95.5%, which were significantly different. (5) But no correlations were found between the positive expression rate of E2F-1 in cases with lymph nodes metastasis(79.5%) and that in those without lymph nodes metastasis(72.0%)( P>0.05). ⑤There was no different between invaded pleura or surrounding tissue group and no invaded visceral pleura group, the positive expression rate of E2F-1 in invaded pleura or surrounding tissue group was 78.6%, in no invaded visceral pleura group was 75.0%. Conclusion The results suggest that transcription factor E2F-1 is excess expression in primary lung carcinoma, the expression of transcription factor E2F-1 was closely associated with clinical pathological types and differentiation degree, but had no relation to clinical stage, whether or not tumor lymph node metastasis and invaded pleura or surrounding tissue. Abnormal expression of transcription factor E2F-1 may be related to the lung tumorigenesis and progression, and may play an important role.更多还原