【作者】 朱海华；

【导师】 陈晖；

【作者基本信息】 浙江大学 ， 口腔医学， 2007， 硕士

【摘要】 [目的]近几年研究表明，细菌和病毒感染引起的炎症反应与冠心病发病有一定关系，其中牙周炎是重要的组成部分。细胞因子是重要的调节细胞和体液炎症反应的蛋白，血清和龈沟液中某些细胞因子的浓度常作为冠心病和牙周病活动性指标，与治疗有密切的关系。本文应用悬液芯片仪检测血清和龈沟液细胞因子水平，分析牙周炎和冠心病的相关性。[方法]共选择40名受检者，分成4组，分别为健康对照组(H)、牙周炎组(P)、冠心病组(C)、冠心病合并牙周炎组(P／C)。对所有受检者进行牙周检查，记录牙周袋深度，松动度等指标，并采取血清和龈沟液标本，应用Bio-Plex悬液芯片仪检测血清和龈沟液中细胞因子IL-1β、MCP-1、VEGF和PDGF水平。[结果]P组、C组、P／C组的血清和龈沟液中IL-1β水平明显高于H组(P＜0.05)；P组、C组、P／C组的血清MCP-1水平明显高于H组(P＜0.05)，而龈沟液水平未体现出差异；P组、C组、P／C组的血清VEGF的浓度低于H组，而龈沟液中VEGF的浓度高于正常组；龈沟液和血清中PDGF的浓度在各组之间没有统计学意义上的差异。[结论]牙周病和冠心病之间可能通过血清和龈沟液中细胞因子水平的变化，相互影响疾病的发展变化过程，其中IL-1β和MCP-1在二种疾病的发生发展中发挥了关键作用，这二种细胞因子水平变化可作为反映牙周病和冠心病病变活跃性变化的重要标记物。更多还原

【Abstract】 [Objective]In the recent years, some reports raised the hypothesis that the incidence and progression of CHD was associated with chronic infections, including periodontal inflammation. Oral inflammation maybe a risk factor of CHD. Cytokines are important cell signalling proteins and mediate a wide range of physiological responses. The level of cytokines in serum and gingival crevical fluid were regarded as markers that contribute to elucidating novel mechanisms of periodontitis and CHD. They provided useful information for the diagnosis and treatment of the two diseases.To study shared etiological factors between CHD and periodontitis, we identified and quantified four kinds of cytokines (IL-1β, MCP-1, VEGF and PDGF) in serum and gingival crevicular fluid (GCF) by Suspension Array System.[Methods]40 subjects were divided into 4 groups, one of which suffered from periodontitis but without CHD (P), and the second group was made of 10 CHD patients with no periodontitis(C), while the third was 10 patients suffering from periodontitis and CHD(P/C) and the fourth was healthy volunteers(H). All of them underwent a comprehensive periodontal examination, such as pocket probing depth and tooth mobility et al. GCF was collected from all individuals, and blood samples were withdrawn on the time of GCF sampling. The four kinds of Cytokines’ levels in GCF and serum samples were detected by Bio-Plex Suspension Assay System.[Results]The IL-1β level in serum and GCF from all patients (including P group, C group, PC group) was higher than healthy subjects (P<0.05). And so was observed for MCP-1 level in serum, however, the level of MCP-1 in GCF had no difference between patients and healthy volunteers. The VEGF concentration in serum of P group, C group, PC group was lower than H group, on the contrary, the VEGF concentration in GCF of the patients was higher than H group. There were no significant differences of the level of PDGF in serum and GCF among four groups[Conclusions]It was concluded that PD and CHD had effect on each other’s progress depending on the changing level of cytokines. Furthermore, elevated levels of IL-1β and MCP-1 in serum and GCF could serve as a reliable marker that reflected the inflammatory activity of PD and CHD.更多还原