【作者】 柳叶；

【导师】 吕秀阳；

【作者基本信息】 浙江大学 ， 化学工程， 2006， 硕士

【摘要】 高纯度磷脂作为一种优良的表面活性剂和乳化剂已经被广泛应用于食品、医药、化妆品等行业。目前国内高纯度磷脂产品尚依赖进口，对其需求也在逐年增加。因此，迫切需要开发出适合我国国情的高纯度磷脂的制备方法。针对我国鸡蛋资源非常丰富但是利用率低下的现状，本课题组一直致力于高纯度蛋黄磷脂的制备研究并且取得了较大的进展。本文在课题组已有研究的基础上，以“氧化铝柱层析分离纯化磷脂的工艺优化”为目标，考察了不同洗脱条件对磷脂在氧化铝柱上的洗脱特性的影响，并进一步进行了氧化铝再生以及工艺连续上样的实验研究，建立并且优化了氧化铝柱层析分离纯化卵磷脂(Phosphatidylcholine，简称PC)和脑磷脂(Phosphatidylethanolamine，简称PE)的完整工艺。系统考察了甲醇—氯仿；甲醇—氯仿—三乙胺；甲醇—氨水；和甲醇—氯仿—氨水洗脱体系对氧化铝分离纯化磷脂工艺的影响。得到最佳的洗脱液组成为甲醇：氯仿：氨水=80：120：10(v／v)。在此条件下，PC和PE的回收率均达到98％以上，理论产量分别为0.31g／g吸附剂和0.04g／g吸附剂。对不同洗脱条件下氧化铝的再生以及工艺的连续上样进行了实验研究。结果表明：在洗脱液为甲醇：氯仿=1：4(v／v)的条件下，PC和PE回收率分别达到99.8％和86.8％，理论产量分别达到0.32g／g吸附剂和0.03g／g吸附剂，再次上样量和三次上样量分别达到首次上样量的80.2％和79.7％，并可同时得到纯度大于95％的PC产品和纯度大于80％的PE产品。在有机溶剂和碱组成的洗脱液体系中，再生和连续上样效果最佳的是甲醇：氯仿：氨水=80：120：10(v／v)的洗脱液。在此条件下，再次上样量为10.85g，达到首次上样量的94.7％。对磷脂在不同溶剂中的稳定性进行了研究。研究结果表明，磷脂在氨水或者三乙胺存在的有机溶剂中是不稳定的。本文的研究建立了氧化铝柱层析分离纯化高纯度磷脂的完整工艺，该工艺具有分离效果好、工艺简单、投资少、处理量较大等优点。研究结果为高纯度卵磷脂和脑磷脂的制备工艺工业化提供了重要的基础数据。更多还原

【Abstract】 High-purity phosphohpids are widely used in the fields of food, pharmacy, cosmetic and so on because of the special surface activity and emulsification. .At present most of this production is imported in China and the requirement enhances every year. So it is necessary to develop the preparation of high-purity phospholipids. The research has been doing and great process has been made in our lab.Aiming at the process optimization for simultaneous purification of PC and PE by alumina column chromatography, the effect of different elution phase on the elution characteristic of PC (Phosphatidylcholine) and PE (Phosphatidyl-ethanolamine) on aluminium oxide column was studied. The regeneration of alumina oxide and the contiunous loading of the process were experimented. The process was developed and optimized absolutely.The elution effect of chloroform-methanol, methanol-chloroform-triethylamine, methanol-ammonia and methanol-chloroform-ammonia on the process for simultaneous purification of PC and PE by alumina column chromatography was studied. The optimized proportion of elution phase is methanol: chloroform:ammonia = 80 ∶120∶10 (v/v) and under this condition the recoveries of PC and PEwere both beyond 98% and the theoretical output of PC and PE were 0.31g/g sorbent and 0.04 g/g sorbent.The regeneration and continous loading were experimented and under the condition of elution phase 80% chloroform-methanol the recoveries of PC and PE were 99.8% and 86.8%; the theoretical outputs were 0.32 g/g sorbent and 0.03 g/g sorbent, the second and third loading amount were as much as the 80.2% and 79.7% of the firet loading amount. PC and PE with purities over 95% and 80% could beobtained. Under the elution condition of methanol: chloroform: ammonia =80 ∶120 ∶10 (v/v), the second loading amount was 10.85g which was 94.7% of the fist loading amount.Based on the former research, the effect of the different elution phases on lecition stability was studied. Experimental results showed that PC and PE were unstable in the solvent with ammonia or triethylamine. The result was valuable for the following work and industrial practice.The process for simultaneous purification of PC and PE by alumina column chromatography was developed and optimized which has satisfied effect, simple techniques, less investment and large amount loading. The work will provide important basic data for industrial preparation of high-purity phospholipids with alumina column chromatography.更多还原