【作者】 樊宇平；

【导师】 蔡谞；

【作者基本信息】 中国人民解放军军医进修学院 ， 外科学， 2007， 硕士

【摘要】 目的：本课题利用中空多孔金属支架为骨组织再生工程构架，观察体外骨髓基质干细胞(bone marrow mesenchymal stem cells，BMSc)在支架内、外的分化生长情况以及成骨的可能性，并探讨体外应用骨诱导因子—骨形态发生蛋白—2(recombined human bone morphogenetic protein-2，rhBMP-2)提高成骨活性，促进金属假体与骨质相对一体化结合的可行性，为研制中空多孔人工关节假体或其他骨组织再生骨植入物提供基础实验依据。希望实验结果能够为生物固定的人工关节设计方法的改进提供一些理论及实验依据。方法：自行设计中空金属试件，采用中空金属试件(A组)、中空金属试件假体+脱钙骨基质(decalcified bone matrix DBM)(B组)、中空金属试件+DBM+成骨诱导液(C组)、中空金属假体+DBM+rhBMP-2培养液(D组)，金属试件均为HA涂层，于BMSc混悬液中加10％胎牛血清DMEM培养液培养，在2、4周观察点取材，通过倒置相差显微镜、扫描电镜、成骨细胞表型检测等方法对中空金属试件表面、脱钙骨基质BMSc生长以及成骨分化进行观察、鉴定。使用SPSS15.0软件包进行统计分析，计量资料以均数±标准差表示，组间同一时间各指标比较采用组间方差分析。结果：实验发现：倒置相差显微镜、扫描电镜等方法显示中空金属试件表面及试件内三维细胞支架上BMSc细胞黏附正常，经成骨诱导后转为成骨细胞，并形成工程化组织。在实验设计的两种骨诱导方式中，成骨细胞的表达以成骨诱导液为明显，在统计学意义上其他对照组有明显差异。结论：一、体外DMSc经诱导向成骨细胞分化，并可在中空金属试件的空间结构中形成组织块。二、中空金属假体的设计思路是可行的，在体外可以观察到BMSc在金属腔壁与腔内细胞支架之间紧密结合，并且在无细胞支架的情况下，1mm孔洞可以形成细胞间的直接连接。因此中空结构的假体是一个值得探索的方向，希望对生物复合型假体的改进具有一定的指导意义。更多还原

【Abstract】 Objective: Histological, histomorphological analysis were used to analyze the bony in-growth conditions in the interface of bone and prosthesis and inside hollow prosthesis of 4 groups. We hope that the results of our research can provide the clinical using new hollow prosthesis to improve biological fixation of artificial joint with some theoretical and experimental data.Methods: We designed hollow prosthesis by self, and divided it into 5 group: hollow prosthesis (A group), hollow prosthesis with DBM (B group), hollow prosthesis with DBM in osteogenesis inducing nutrient liquid (D group), hollow prosthesis with DBM combined with rhBMP-2(D group). All hollow prostheses coated with hydroxyapatite. Then BMSc at the surface of the hollow prostheses and in the DBM was researched by inverted phase contrast microscope, scanning electron microscope, osteoblast phaenotype detection after 2and 4 weeks.Measurement data were expressed as means+SD(x±s) and analyzed by t Test.Results: Inverted phase contrast microscope and scanning electron microscope showed that BMSc at the surface and the hole of the hollow prosthese and in the DBM 3—D cytoskeleton can adhere normally and transform into osteoblast by inducing and form engineering tissue. Osteoblast phaenotype detection after 2 weeks shows the results: the present of osteoblast of C group is significant difference with other groups statisticallyConclusion : 1.BMSc at the surface and the hole of the hollow prostheses and in the DBM 3—D cytoskeleton can adhere normally and transform into osteoblastby inducing and form engineering tissue in vitro.2.the desigen of the hollow prostheses is feasible. It shows in vitro that BMSc at the wall of the prosthesis can combine with the DBM 3—D cytoskeleton and BMSc in the 1 mm hole of the prosthesis without 3—D cytoskeleton can also connect directly.So the hollow prosthesis is worthy with more researching, having some guidance in clinic.更多还原