【作者】 余少欢；

【导师】 王自能；

【作者基本信息】 暨南大学 ， 妇产科学， 2006， 硕士

【摘要】 目的 利用免疫组织化学及图像分析技术，定位在胎儿生长受限(FGR)和正常足月胎盘组织中的白血病抑制因子(LIF)及瘦素(Leptin)，探讨它们的相关性及与胎儿生长发育的关系。 方法 取人正常足月分娩胎盘与胎儿生长受限胎盘各15例，行常规HE染色对胎盘组织行形态学研究，借助免疫组化SP法及图像分析技术定量分析各例胎盘组织中LIF及Leptin在绒毛滋养层的的表达。 结果 1．与正常妊娠组胎盘比较，FGR组胎盘绒毛发育欠佳，绒毛血管扩张不良，细胞滋养细胞增生，血管合体膜增厚。 2．LIF主要表达于绒毛滋养细胞和血管内皮细胞胞浆中；Leptin主要表达于胎盘合体滋养层细胞的胞浆中。更多还原

【Abstract】 PurposeTo study the expression and the localization of leukemia inhibitory factor (LIF) and leptin in placenta from normal pregnancy and fetal growth restriction (FGR).MethodsPlacental samples obtained from 30 women were divided into 2 groups, FGR group (n=15) and control group (n=15). Tissue samples were fixed in formalin, embedded in paraffin and processed for routine histological examination and immunohistochemical identification of LIF and Leptin expression.Results1.FGR tissue sections compared with control group, showed a poor development on placental villus, villous capillaries were not dilated, hyperplasia of the cytotrophoblasts increased the thickness of vasculosyncytial membrane.2.LIF mainly expressed in placenta villous trophoblasts and fetal vasular endothelium; Leptin expressed on the placenta villous syncytiotrophoblast.更多还原