【作者】 吕虹；

【导师】 陈华德；

【作者基本信息】 浙江中医药大学 ， 针灸推拿学， 2006， 硕士

【摘要】 目的 观察不同频率电针对学习记忆能力偏低大鼠空间学习记忆能力的治疗作用，筛选改善学习记忆能力偏低大鼠空间学习记忆能力的最佳电针频率。同时观察电针对兴奋性氨基酸含量及NMDA受体NR2B亚单位蛋白表达的干预作用，初步探讨电针影响学习记忆能力的部分作用机理。 方法 把通过水迷宫筛选出的学习记忆能力偏低大鼠作为本实验动物模型，设立空白对照组和4个不同频率的电针治疗组：2Hz组、15Hz组、100Hz组、2／100Hz组。电针大鼠“百会”穴和“哑门”穴，每天1次，每次30min，连续治疗18天。通过水迷宫检测各组大鼠空间学习能力和记忆能力、使用氨基酸自动分析仪检测脑组织中兴奋性氨基酸含量、应用免疫印迹方法检测NR2B的蛋白表达。 结果 (1)各电针治疗组空间学习能力均有所改善，其中2Hz组改善较为显著(P＜0.05)，并且持久；(2)2Hz组、100Hz组、2／100Hz组空间记忆能力均有改善，2Hz组改善作用较为明显(P＜0.05)；(3)各电针治疗组兴奋性氨基酸含量均较空白对照组低，其中2Hz组含量最低；(4)各电针治疗组NR2B蛋白表达均较空白对照组低，其中2Hz组最低。 结论 (1)电针能有效改善学习记忆能力偏低大鼠的空间学习记忆能力；(2)改善学习记忆能力偏低大鼠空间学习能力和记忆能力的最佳电针频率是2Hz；(3)本实验所选择的大鼠其学习记忆能力偏低可能与大鼠脑中兴奋性氨基酸含量较多有关；(4)电针改善学习记忆能力偏低大鼠的空间学习记忆能力可能与减少兴奋性氨基酸的含量，抑制了兴奋性氨基酸对NMDA受体过度激活所引起的毒性作用有关；(5)电针改善学习记忆能力偏低大鼠空间学习记忆能力的确切机制还有待于进一步深入研究。更多还原

【Abstract】 Objective To observe the effect of electro-acupuncture(EA) of different frequency on affecting ability of spatial learning and memorizing in rats with deficiency of learning and memorizing. To select the best frequency of EA for improving spatial learning and memorizing in rats with deficiency of learning and memorizing. Meanwhile observe the intervention of EA in the content of excitatory amino acid and protein expression of NR2B subunit in rats, so as to discuss the mechanism of EA on affecting learning and memorizing in rats.Methods The model rats with deficiency of learning and memorizing chose by Morris water maze were divided into one blank control group and four EA therapeutic groups including the 2Hz group, the 15Hz group, the 100Hz group and the 2/100Hz group. Needled Baihui and Yamen acupoints in rats with the electro-acupuncture once a day and remained the needles for 30min per time, gave a continuous course treatment for 18 days. We observed the spatial learning and memorizing in all rats by Morris water maze, detected excitatory amino acid content by automatic amino acid analyzer and protein expressionof NR2B subunit in brain tissue with immunoblotting in all rats.Results First, the spatial learning in all EA therapeutic groups was improved.The improvement in the 2Hz group was better than the others (P<0.05). Second, the spatial memorizing in the 2Hz group, the 100Hz group and the更多还原