【作者】 张敏；

【导师】 张煦；

【作者基本信息】 兰州大学 ， 病理学与病理生理学， 2006， 硕士

【摘要】 目的：检测MMP-7及TIMP-1、Survivin、Ki-67在子宫腺肌病异位内膜、在位内膜和对照组内膜中的表达情况，以了解侵袭因素与细胞凋亡、增殖在子宫腺肌病发病过程中的作用及其相关性，并探讨其在临床早期诊断和治疗中的应用价值。 方法：采用免疫组化S-P法检测子宫腺肌病异位内膜、在位内膜及正常对照组内膜中MMP-7及TIMP-1，Survivin，Ki-67的表达。 结果：1．MMP-7在子宫腺肌病异位内膜、在位内膜的表达显著高于正常内膜，差异有显著意义(P＜0.05)，且这种表达的差异主要体现在分泌期子宫内膜(P＜0.05)。TIMP-1在正常子宫内膜的表达高于腺肌病异位内膜、在位内膜，差异有显著意义(P＜0.05)，且这种表达的差异主要体现在分泌期子宫内膜(P＜0.05)。在正常子宫内膜组织中MMP-7与TIMP-1的表达之间有负相关关系(P＜0.05)，在腺肌病异位内膜、在位内膜中二者不具有相关性(P＞0.05)。 2．子宫腺肌病异位内膜中Survivin的表达显著高于在位内膜及正常内膜(P＜0.05)，且这种表达的差异主要体现在增生期子宫内膜(P＜0.05)，而在位内膜与正常内膜比较，差异无显著性意义(P＞0.05)。在腺肌病异位内膜组织中Survivin与MMP-7的表达之间有正相关关系(P＜0.05)； 3．在位内膜与正常内膜中Ki-67的表达有周期性变化，增生期显著强于分泌期(P＜0.05)。而在子宫腺肌病异位内膜中，Ki-67的表达在分泌期和增生期差异无显著性(P＞0.05)。在腺肌病异位内膜组织中Ki-67与Survivin、MMP-7的表达之间有正相关关系(P＜0.05)； 4．子宫腺肌病患者的痛经程度与异位内膜中MMP-7的表达之间存在正相关性(P＜0.05)，而与Survivin、Ki-67不存在相关性(P＞0.05)。 结论：MMP-7及TIMP-1的比例失调，可能是子宫腺肌病的异位内膜、在位内更多还原

【Abstract】 Objective: To investigate the expression of matrix metalloproteinase-7(MMP-7), tissue inhibitor of matrix metalloproteinase-1(TIMP-1), Survivin and Ki-67 in eutopic endometrium, ectopic endometrium of adenomyosis and normal human endometrium in order to study the function and relativity of invasion ,apoptosis and proliferation in the course of adenomyosis . Its early clinical diagnosis and the treatment application are discussed.Method: Specimens of normal endometrium , eutopic endometrium of adenomyosis and ectopic endometrium of adenomyosis were obtained for immunohistochemical MMP-7 , TIMP-1, Survivin and Ki-67. Results:1. The expression of MMP-7 in eutopic endometrial tissue and ectopic endometrial tissue of adenomyosis was significantly higher than that of normal endometrium(P<0. 05). The discrepancy was mainly reflected in secretory phase. TIMP-1 in normal endometrium was expressed significantly higher than eutopic endometrial tissue and ectopic endometrial tissue of adenomyosis (P<0. 05). The correlation between the expression of MMP-7 and TIMP-1 in normal endometrium was negative (P<0.05), while no correlation had been found in eutopic endometrial tissue and ectopic endometrial tissue of adenomyosis(P>0. 05). 2. The expression of Survivin in ectopic endometrium of adenomyosis was significantly higher than that of normal endometrium and eutopic endometrium of adenomyosis, as it was seen mostly in proliferative phase(P<0.05). No significant diferences between the eutopic endometrium of adenomyosis and of normal endometrium were observed (P>0. 05). There更多还原