【作者】 乔楠；

【导师】 彭健；

【作者基本信息】 华中农业大学 ， 动物遗传育种与繁殖， 2006， 硕士

【摘要】 组织两次试验分别研究在生长肥育猪麦麸和双低菜粕两种低能日粮中添加木聚糖酶，通过饲养试验和消化试验考察酶对两类日粮养分消化率和有效能改善的效果。 试验一选择70日龄、体重27Kg左右的杜长大三元杂交仔猪72头，采用3×3因子设计，研究在以玉米为主的生长肥育猪(27-100kg)日粮中木聚糖酶的添加水平，小麦麸的含量以及两者的互作对动物性能和养分消化率的影响。麦麸含量为0、10％、20％三个水平，酶添加水平为0、0.05％、0.1％，共9个处理，每个处理8头猪，单栏饲养。试验结果如下： 1、与未添加麦麸组相比，添加麦麸10％和20％，对生长肥育猪的日增重和料肉比无显著影响(P＞0.05)；在麦麸水平为0、10％和20％日粮中，添加0.05％木聚糖酶组使全期采食量相比对照组分别提高了8.6％(P＞0.05)、9.6％(P＜0.05)和7.6％(P＞0.05)。 2、麦麸水平的升高极显著降低了干物质、粗蛋白和中性洗涤纤维的消化率(P＜0.01)；添加木聚糖酶极显著提高了不同麦麸水平的粗蛋白和中性洗涤纤维的消化率(P＜0.01)，显著提高了日粮的消化能(P＜0.05)。总体看来，木聚糖酶和麦麸水平表现出极显著的互作关系(P＜0.01)。在麦麸水平为10％的日粮中，添加木聚糖酶0.05％显著极提高了各项营养物质的消化率(P＜0.01)，对日粮消化能的提高幅度最大为100Kcal／kg。 试验二选择30kg左右杜长大三元杂交猪200头，采用2×2因子设计，研究木聚糖酶在生长肥育猪(30-90kg)不同双低菜粕日粮类型中的作用效果，双低菜粕分脱皮处理和未脱皮处理两种，木聚糖酶分不添加和添加0.05％两个水平，设玉米豆粕型日粮为对照组，共5个处理，每个处理3个重复栏，其中2个大栏，1个小栏，大栏每栏15头、小栏每栏10头。试验结果如下： 1、正对照组的前期日增重相比脱皮组和脱皮加酶组分别组提高了7.1％和6.5％(P＜0.05)，脱皮加酶组的采食量和料肉比相比未脱皮组低了9.6％和7.2％(P＜0.05)。 2、在脱皮处理组中，添加0.05％的木聚糖酶使生长肥育猪的平均日增重较对照组提高了2.0％(P＞0.05)，料肉比降低了5.2％(P＞0.05)；粗蛋白消化率提高了11.79％(P＜0.05)，中性洗涤纤维消化率提高了11.44％(P＞0.05)，消化能提高了300Kcal／kg(P＜0.05)。 上述研究结果表明：在生长肥育猪麦麸型和双低菜粕日粮型两种低能日粮中，添加木聚糖酶可以提高生长肥育猪的生长性能，改善养分消化率以及提高可消化能。日粮的能量水平、底物的含量以及酶的添加量都会影响酶的作用效果。更多还原

【Abstract】 In this study two feeding and digestibility trials were conducted to evaluate the effect of xylanase supplementation on bioavailible energy in wheat bran and double-low rapeseed meal of growing and finishing pigs.Experiment 1: A total of 72 Large yorkshirexLandrace crossbred pigs,average initial BW of 27.1 kg, were allocated to 9 dietary treatments and with 8 replicates each arranged as a 3 x 3 factorial design to study the interactions between xylanase and wheat bran in corn-based diets for growing-finishing pigs.Experimental diets consisted of 0 ,10% and 20% wheat bran. The xylanse inclusion levels is 0, 0.05 % and 0.10%. The experimental diets were aimed to be sufficient in crude protein and limiting in energy content with wheat bran as the adjustment for energy. The results suggested:1. Wheat bran proportions increased from 0 to 20% had no significant(P>0.05) effect on feed intake, growth rate and feed conversion ratio. Supplemental 0.05% xylanase in comparison to the control, ADFI increased 8.6%(P>0.05),9.6% (P<0.05)and 7.6%(P>0.05), respectively.2. As the increase of the wheat bran contents in growing-finishing pig diets, significantly decreased apparent fecal digestibility of DM(P<0.05),CP(P<0.01) and NDF(P<0.01). Supplemental xylanase in maize-based diets containing different level of wheat bran for growing-finishing pigs significantly(P<0.01) improved apparent fecal digestibility of CP and NDF and significantly(P<0.01) improved diets DE(P<0.05).The interaction of xylanase and wheat bran significantly(P<0.01) influenced apparent fecal digestibility of DM,CP and NDF. Supplemental 0.05% xylanase in diet with 10% wheat bran improved 100Kcal/kg DE,which is the best effect.Experiment 2: A total of 200 Large yorkshirexLandrace crossbred pigs,average initial BW of 30 kg, were allocated to 5 dietary treatments and each treatment with 3 replicate pens:2 large pens of 15 pigs per pen and 1 samll pen of 10 pigs, arranged as a 2 × 2 factorial design to study the effect of xylanase on dehulled DLRM and DLRM based diets for growing-finishing pigs. The result was as follows:1、 Compared with the dehulled DLRM treatment with and without xylanase addition, positive control increased ADG 7.1% and 6.5% (P<0.05), respectively. Compared with the DLRM treatment, supplemental xylanase in dehulled DLRM treatment decreased ADFI and FCR by 9.6% and 7.2%(P<0.05).2、 Compared with the dehulled DLRM treatment, supplemental xylanase increased ADG by 2.2%, decreased FCR by 5.2%; apparent fecal digestibility of CP and NDF increased by 11.79%(P<0.05)and 11.44%(P>0.05),respectively;DE increased by更多还原