CTLA4-1gG在糖尿病大鼠胰腺移植中的作用研究

【作者】 李军杰；

【导师】 张肇达；

【作者基本信息】 四川大学 ， 外科学， 2006， 硕士

【摘要】 【目的】探讨细胞毒性T淋巴细胞相关抗原4免疫蛋白(cytotoxicity T lymphocyte-associated 4-immunoglobulin G，CTLA4-IgG)对同种异体大鼠胰腺移植排斥反应的影响。 【方法】 予Wistar大鼠静脉注射链脲霉素(streptozotocin，STZ)诱导制成Ⅰ型糖尿病模型，以其为胰腺移植的受者，并随机分为对照组和实验组，各16例。正常SD大鼠为供者。整块切取供者胰十二指肠，保留带有腹腔干和肠系膜上动脉的腹主动脉段。切除受者左肾，保留右肾，将供者门静脉和受者左肾静脉应用套管法行端端吻合；供者腹主动脉段与受者肾下腹主动脉行端侧吻合；供者十二指肠与受者空肠行侧侧吻合。实验组分别于术中、术后1—7d腹腔注射CTLA4-IgG；对照组分别于术中术后1—7d腹腔注射无菌柠檬酸缓冲液。两组受者术后1—7d每日监测血糖观察移植物功能性存活情况，ELISA检测术后3、7d血清IL-2浓度，术后7d取移植物标本行病理学检查并处死。 【结果】 胰腺移植后糖尿病大鼠受者血糖恢复正常，术后6d，对照组血糖10.6±1.87mmol／L，实验组血糖8.0±0.60mmol／L，两组相比差异有统计学意义(P＜0.01)。实验组术后3、7d血清IL-2浓度分别为更多还原

【Abstract】 Objective : To evaluate the effect of CTLA4-IgG on the immunological tolerance of pancreaticoduodenal transplantation model in allogenic rats.Methods: The diabetes Wistar rats as recipients were induced by intravenous administration streptozotocin (STZ) in a single dose of 60mg/ kg of body weight via penis dorsal vein. The diabetes rats were randomly assigned to control group (n = 16) and transplantation group (n=16). Pancreaticoduodenal transplantations were done with the SD rats as donors,then the graft including duodenum and pancreas were harvested enbloc, while the abdominal aorta segment including the coeliac artery and mesentery artery were kept intact. In the recipient operations, the left kidneys was removed while the right kidney was kept intact, the recipient left renal vein was anastomosed with the donor portal vein by end-to-end fashion, the recipient abdominal aorta was anastomosed with the donor proximal end of donor abdominal aorta by lateral-to-end fashion, and the recipient small intestine was anastomosed with the donor duodenum by lateral-to-lateral fashion. The operation group were administered via celiac injection with CTLA4-IgG during operation and after operation(from the 1st day to 7th day) The control group were administered via celiac injection with axenic citric acid puffer solution in the same way as the operation group .Blood glucose of the two recipient groups were investigated daily after transplantation for 7 days, The graft functional survive interval (GFSI) was recorded synchronously. The concentration of IL-2 was observed with ELISA on the 3rd, 7th day after operation. Pancreas tissue biopsies were done on the 3rd,更多还原