【作者】 杨鹏；

【导师】 俞理；

【作者基本信息】 中国科学院研究生院（渗流流体力学研究所） ， 流体力学， 2006， 硕士

【摘要】 筛选性能优异的菌种是微生物采油的关键技术之一。菌种的好坏直接关系到矿场应用的效果和微生物采油技术发展的前景。 本文研究的是一种通过原生质体融合技术构建出的细胞工程菌。通过室内生物化学实验、室内物理模拟实验、可视化微观模拟实验和环境扫描电镜方法比较了原生质体融合菌FH菌和其亲本菌JD菌生理生化特性及油藏适应性评价，优选了FH菌的培养基，分析了FH菌种在多孔介质中的运移能力、封堵能力及在非均质地层中的调剖驱油效果、研究了FH菌的调剖驱油机理。在比较系统的实验研究基础上，得到了一些规律性的认识。 研究表明：FH菌比其中的亲本菌JD菌能更好的适应油藏条件；FH菌在多孔介质中能够很好的封堵大孔道，调整水驱的吸水剖面，增加非均质油层的采收率。并通过光刻玻璃微观模型及先进的环境扫描电镜从微观角度证实FH菌在多孔介质中能够生长代谢产生生物多糖和生物气体。生物多糖部分在水相中，部分与菌体共同吸附在岩石颗粒的表面改变了孔隙半径，可动的生物气体能够有效驱替原油，不可动生物气体在大孔道中可以起到贾敏效应，这些因素都提高了调剖驱油的效果。 通过本文研究表明，通过原生质体融合技术获得的FH菌在多孔介质中代谢产生的生物多糖及生物气体能够很好的起到调剖堵水提高原油采收率的作用，从而进一步能够为油田的微生物调剖矿场试验和实际生产服务。更多还原

【Abstract】 It is one of the pivotal technology to select excellent performance a strain in MEOR. It will influence on the effect of application in oil field and developmental foreground in MEOR.In this paper, the strain is cell engineering bacteria, which constructed by the way of protoplast fusion. The methods of biochemistry experiment, laboratory physical simulation experiment, micro-imitated glass model and environment scanning electron microscope (ESEM) compared the physiology and biochemistry characteristic and the growing condition of the strain adapting oil formation of the bacterium FH, which obtained by the way of protoplast fusion, to the bacterium JD, which is one of parents strains, selected the culture medium of the bacterium FH, analyzed the transport and shut-off capacity of the bacterium FH in porous media, and recovery efficiency of water driving oil in nonhomogeneous formation, studied the profile modification and oil displacement mechanism. Furthermore, some systematic conclusions were also acquired.The results show that the bacterium FH can metabolize in oil formation rather than one of parents strains, which notated as bacterium JD, show that the microbial polysaccharide, which bacterium FH producing in porous media, commendably can close off big channel, can adjust injection profile, can enhance recovery efficiency of water driving oil in nonhomogeneous formation. The experiment by the way of micro-visual analysis with micro-imitated glass model and environment scanning electron microscope (ESEM) indicated the bacterium FH can produce microbial polysaccharide and microbial gas in porous media. Some polysaccharide is in the water phase, some adsorb surface of rock grain with some thallus bring on reducing the pore radius. Movable microbial gas can be capable of drive oil, and immovable更多还原