【作者】 丁虹茹；

【导师】 朱玮； 高锦明；

【作者基本信息】 西北农林科技大学 ， 植物学， 2006， 硕士

【摘要】 本文以微生物发酵学,植物化学和现代化学分离理论为基础,在白绒红蛋鸟巢菌发酵液及菌丝体提取物抑菌活性跟踪检测的前提下,对其菌丝体乙酸乙酯提取物的化学成分进行了分离纯化,并对纯化合物进行了活性测定。研究结果表明:在浓度为12.5mg/mL时,发酵液的乙酸乙酯提取物和菌丝体的乙酸乙酯提取物对金黄色葡萄球菌(Staphylococcus aureus)的抑制作用相同。而浓度为6.25mg/mL时,发酵液的乙酸乙酯提取物对巨大芽孢杆菌(Bacillus megaterium)活性显著,对灵杆菌(Bacillus prodigious)无活性,但菌丝体的乙酸乙酯提取物对灵杆菌活性显著,对巨大芽孢杆菌无活性。在浓度为25mg/mL时,发酵液的乙酸乙酯提取物和菌丝体的乙酸乙酯提取物对枯草杆菌(Bacillus subtilis)均有显著活性。发酵液的乙酸乙酯提取物对大肠杆菌(Escherichia coli)和腊状芽孢杆菌(Bacillus subtilis)的最小抑制浓度(MIC)分别为25mg/mL和12.5mg/mL,而菌丝体的乙酸乙酯提取物对大肠杆菌和腊状芽孢杆菌无活性。发酵液的乙酸乙酯提取物和菌丝体的乙酸乙酯提取物对变形杆菌(Proteus species)均无活性。发酵液的乙酸乙酯提取物对黄瓜炭疽( Colletotrichum lagenarium)的MIC为6.25mg/mL,而菌丝体的乙酸乙酯提取物对黄瓜炭疽无活性。在浓度为25mg/mL时,发酵液的乙酸乙酯提取物和菌丝体的乙酸乙酯提取物对烟草赤星(Alternaria alternata)和白菜黑斑(Alternaria brassicae)均有活性。菌丝体的乙酸乙酯提取物对辣椒晚疫(Phytophthora capsici)和小麦纹枯(Ceratobasidium corniger)的活性比发酵液的乙酸乙酯提取物强。在浓度为12.5mg/mL时,发酵液的乙酸乙酯提取物和菌丝体的乙酸乙酯提取物对棉花黄萎(Verticilliu dahliae)均有活性。在浓度为25mg/mL时,发酵液的乙酸乙酯提取物和菌丝体的乙酸乙酯提取物对霉菌无活性。发酵液的正丁醇提取物和菌丝体的正丁醇提取物,在浓度为25mg/mL时,对细菌和植物病原菌均无活性。当浓度为12.5mg/mL时,发酵液的正丁醇提取物和菌丝体的正丁醇提取物对青霉(Penicilliumsp.)有抑制作用。发酵液的正丁醇提取物对土曲霉(Aspergillus terreusP)、绳状青霉(Penicillium funiculosum)、产黄青霉(Penicillium chysogenum)和宛氏拟青霉(Paecliomyces variotii Bainier)的活性比菌丝体的正丁醇提取物活性强。发酵液的正更多还原

【Abstract】 This thesis, guided by microbial zymology, phytochemistry and theory of chemical separation, deals with isolation and purification of chemical composition of ethyl acetate extractive from mycelia of Nidula niveotomentosa and deals with determination of the bacteriostasis of the chemical composition, after the bacteriostasis of the extractive from fermenting liquor and mycelia of Nidula niveotomentosa had been tracked and detected in the elementary stage. The following are the results of the study.Both the 12.5mg/m ethyl acetate extractive of fermenting liquor and mycelia had the same inhibitory effect on Staphylococcus aureus. The 6.25mg/mL ethyl acetate extractive of fermenting liquor was noticeably inhibitory to Bacillus megaterium, with no effect on Bacillus prodigious, but by contrast, The 6.25mg/mL ethyl acetate extractive of mycelia was noticeably inhibitory to Bacillus prodigious with no effect on Bacillus megaterium. Both the ethyl acetate extractive of fermenting liquor and that of mycelia with the same concentration of 25mg/mL were obviously inhibitory to Bacillus subtilis. The MICs of the ethyl acetate extractive of fermenting liquor were respectively 25mg/mLand 12.5mg/mL,when inhibitory to Escherichia coli and Bacillus subtilis. Both ethyl acetate extractives were no inhibitory to Proteus species.The ethyl acetate extractive of fermenting liquor was inhibitory to Colletotrichum lagenarium with its MIC being 6.25mg/mL, while the ethyl acetate extractive of mycelia was not inhibitory to Colletotrichum lagenarium. Both the 25mg/mL ethyl acetate extractives were inhibitory to Alternaria alternata and Alternaria brassicae. Especially, the ethyl acetate extractive of mycelia was more inhibitory to Phytophthora capsici and Ceratobasidium corniger than that of fermenting liquor. Both the ethyl acetate extractives were inhibitory to Verticilliu dahliae.Both the 25mg/mL ethyl acetate extractives were not inhibitory to moulds.Both the 25mg/mL nbutanol extractives of fermenting liquor and mycelia were not inhibitory to bacteria or plant pathogenic bacteria.Three compounds were isolated from the ethyl acetate extractive of mycelia of Nidula更多还原