【作者】 王文泽；

【导师】 李铣； 赵余庆；

【作者基本信息】 沈阳药科大学 ， 药物化学， 2005， 硕士

【摘要】 本论文综合利用硅胶、聚酰胺、葡聚糖凝胶（Sephadex LH-20）柱色谱、制备薄层、重结晶等各种手段，对椒目95％乙醇提取物的化学成分进行系统分离，从中分离得到12个化合物，根据理化性质测定及波谱数据分析，鉴定了其中10个化合物的结构（见Table 2-1），分别为Obtucarbamate A（1）、表儿茶素（2）、槲皮素（3）、24-烯-环阿尔廷酮（4）、辛二酸（5）、硬脂酸（6）、β-谷甾醇（7）、胡萝卜苷（8）、异欧前胡素（9）、异茴芹素（10）。其中化合物1，2，3，4，5，9为首次从该属植物中分离得到。 本论文采用气相色谱法，首次对我国10个不同产地椒目药材中α-亚麻酸的含量进行了比较研究，通过方法学考察证实，精密度、重现性、稳定性良好，加样回收率等符合规定。结果表明：10个产地的样品中，河北、四川产椒目中α-亚麻酸含量较高。本文方法简便，结果可靠，可以用来评价和控制药材的质量，为合理开发利用椒目药用资源提供了参考依据。 本论文对椒目中具有明显抗哮喘作用的椒目油进行了药理活性研究，结果表明：椒目油具有明显的平喘、止咳、抗炎、抗过敏及抗应激作用，其作用机理可能与稳定肥大细胞膜以及减少AA脂氧酶代谢产物白三烯（LTB₄、LTC₄、LTD₄、LTE₄）的生成有关。更多还原

【Abstract】 Twelve compounds have been isolated from the seeds of Zanthoxylom bungeanum Maxim, by open-column, preparative TLC and Sephadex LH-20 column chromatography methods etc. According to their physico-chemical properties and spectroscopic analysis, ten compounds (see Table 2-1) have been identified as Obtucarbamate A (1), epicatechin (2), quercitrin (3), 9,19-cyclolanost-24-en-3-one (4), octanedioic acid (5), stearic acid (6), β-sitosterol (7), daucosterol (8), isoimperatorin (9), isopimpinellin (10). Compounds 1,2,3, 4, 5, 9 were isolated from the genus for the first time.The content of α-linolenic acid was determined in the seeds of Z bungeanum Maxim, from ten habitants in China. The contents of α-linolenic acid the seeds of Z bungeanum Maxim which obtained from Hebei and Sichuan were much more higher . The determination results indicated that the quality of the seeds of Z bungeanum Maxim, could be evaluated and controlled by GC. It was proved that the method was an effective and convenient reference standard for evaluating and controlling the herb of Z bungeanum Maxim.In addition, the results of the pharmacological research showed that the seeds oil of Z bungeanum Maxim. had the effects of anti- asthma, coughing stopping, anti-inflammatory and anti-stress. The mechanism was relative to stabilizing the membrane of mast cell to decrease the amount of LTB4, LTC4, LTD4 and LTE4, which were the metabolites of AA hydrolyzed by lipoxidase.更多还原