【作者】 官涛；

【导师】 王亚平； 王建伟；

【作者基本信息】 重庆医科大学 ， 人体解剖与组织胚胎学， 2006， 硕士

【摘要】 目的白血病是人类好发的恶性肿瘤,是由于造血干细胞增殖分化异常而导致的恶性增殖性疾病。迄今白血病的治疗仍主要采用传统的大剂量联合化疗,但此疗法副作用大,复发率高,而且对正常机体免疫与造血系统有极大的损害。人参总皂苷(TSPG)是人参“补气生血”或促进造血的有效成分。我们既往研究表明:TSPG对造血系统恶性肿瘤的增殖有抑制作用。但TSPG对白血病增殖抑制及诱导分化的机理尚不清楚。本研究采用体外细胞培养技术观察TSPG对K562细胞增殖分化的影响,旨在阐述人参对血细胞发生的调控机制,为研制开发天然中药分化诱导剂提供理论与实验依据。方法1、体外培养白血病细胞株K562。2、采用MTT法、细胞形态观察探讨TSPG对K562细胞增殖的抑更多还原

【Abstract】 ObjectiveLeukemia is a very familiar malignancy in human being. Its pathogeny is due to bone marrow hematopoietic stem cells proliferation and differentiation abnormally. So far, traditional chemotherapy and radiotherapy are still the main therapies, but they have many side effects and the ratio of relapse is very high. What’s more, it greatly damages the normal immunity and hematopoiesis. Total saponins of Panaxginseng (TSPG) is one of the main effective ingredient extracted from Panax Ginseng which plays a vital role in“invigorating qi”or promoting haematopoietic function. Our previous study suggested that TSPG can inhibit the proliferation of leukemia cells. But the molecular biological mechanism for TSPG inhibiting proliferation and inducing differentiation of K562 cells is not clear. By using techniques of cell culture ex-vivo, we can observe the effect of TSPG on proliferation and differentiation of K562 cells. Our purpose is to clarify the modern biological mechanism for TSPG regulating haematopoietic function, and to provide the theoretical basis and the experimental evidence of its clinic application.Methods1, The leukemia cell line (K562) was cultured out of body.更多还原