【作者】 高利娟；

【导师】 蒋代华；

【作者基本信息】 广西大学 ， 植物营养学， 2006， 硕士

【摘要】 微生物-矿物的相互作用是地球上广泛存在的一种相互作用。对环境中营养元素和污染物质的转化等具有重要的影响；土壤矿物是土壤的骨架，粘土矿物是土壤中最具活性的颗粒，细菌作为土壤中种类最多、数量最大的一类微生物，它与粘土矿物的相互作用对土壤物理、化学以及生物学过程都具有极其重要的影响。为此，本文以土壤中的主要粘土矿物（高岭石、蒙脱石、针铁矿）和土壤中最常见的细菌（芽孢杆菌、假单孢菌）为材料，建立了细菌在三种粘土矿物表面吸附的测定方法；同时研究了影响粘土矿物对细菌吸附的因素，取得的主要结果如下： 1．采用三种蛋白质测定方法对细菌悬液中细菌细胞的蛋白质量进行了测定。结果表明：不管采用那种蛋白质测定方法测定，细菌悬液中细菌细胞的蛋白质量与细菌细胞数量呈极显著正相关(r_Lowry=0.9914^**；r_Bradford=0.9719^**；r_Ninhydrin=0.9553^**)。因此，细菌细胞的数量可以用细菌细胞的蛋白质量来表征。 2．Nycodenz分离液可以有效的分离被矿物吸附的细菌细胞与未被矿物吸附的游离态细菌细胞。但Nycodenz分离液对三种蛋白质测定方法测定细菌细胞蛋白质量的结果均有干扰，而对考马斯亮蓝法的影响最小。因此，在研究粘土矿物与细菌的相互作用时，测定细菌细更多还原

【Abstract】 Interaction between microorganisms and soil minerals widely exists in the earth, which plays a great role in transmutation of nutrient element and organic pollutants in environment including water, soil and gas environment. Soil mineral plays a great role in soil, clay mineral are a active particles. Bacteria are most number in microorganisms. Interaction between microorganisms and clay minerals play a large role in soil physics, chemistry and biology. In the present paper, we selected clay minerals including kaolinite, smectite, and goethite and bacteria including Bacillus thuringiensis and Pseudomonas putida as the material to establish the method for study the interaction between bacteria and clay minerals and to study the effects of ambient factors on interaction between bacteria and clay minerals. The main results are as follows:1. Three methods for determining of concentration of proteins were used to determine the concentration of proteins in solution containing bacteria. As a result: Relationships between the concentration of proteins and the number of bacteria were established, which showed the relationship between the concentration of proteins and the number of bacteria was linear. Therefore, the number of bacteria in solution was used to replace by the concentration of proteins.更多还原