【作者】 吴喜梅；

【导师】 张师前；

【作者基本信息】 山东大学 ， 妇产科学， 2006， 硕士

【摘要】 【目的】 研究MAGE、BAGE、GAGE基因在子宫内膜癌组织、正常子宫内膜组织及子宫内膜癌细胞株KLE中的表达情况，探讨这些肿瘤特异性抗原作为子宫内膜癌免疫治疗特异性靶位的可能性。同时研究5—杂氮脱氧胞嘧啶(DAC)对子宫内膜癌细胞株KLE增殖和肿瘤特异性抗原MAGE、BAGE、GAGE表达的影响。 【方法】 采用逆转录—聚合酶联反应(RT-PCR)技术，检测35例子宫内膜癌组织、15例正常子宫内膜组织及子宫内膜癌细胞株KLE中MAGE-1、MAGE-3、BAGE、GAGE1-2基因mRNA水平的表达情况；同时将处于对数生长期的子宫内膜癌细胞株与5—杂氮脱氧胞嘧啶(DAC)共同培养，应用RT-PCR法检测子宫内膜癌细胞株KLE在DAC作用前后肿瘤特异性抗原基因MAGE、BAGE、GAGE mRNA水平表达情况的变化。以β—actin作为内参照。 【结果】 35例子宫内膜癌组织中，MAGE-1、MAGE-3、BAGE、GAGE1-2表达阳性率分别是46％(16／35)、49％(17／35)、20％(7／35)和26％(9／35)，有77％的标本至少表达其中一种基因。15例正常子宫内膜组织中MAGE-1、MAGE-3、BAGE、GAGE1-2基因表达均为阴性。子宫内膜癌细胞株KLE中BAGE基因表达阳性，而MAGE-1、MAGE-3、和GAGE1-2基因阴性，DAC诱导后肿瘤特异性抗原基因表达增加，MAGE-1出现阳性表达。 【结论】 MAGE、BAGE、GAGE基因表达产物是一种肿瘤特异性抗原，更多还原

【Abstract】 Objective: The aim of this study was to observe the expression of MAGE, BAGE , GAGE genes in endometrial carcinoma , normal endometrium and endometrial carcinoma cell line (KLE), and to evaluate the possibility of using these genes as molecular markers and as the targets of specific immunotherapy for endometrial carcinoma. The other aim of this study was to investigate the effect of 5-aza-2’-deoxycytidine(DAC)in proliferation of endometrial carcinoma cell line and expression of tumor specific antigen MAGE, BAGE, GAGE .Materials and Methods: Samaples of 35 endometrial carcinoma, 15 normal endometrial tissues and endometrial carcinoma cell line were examined with reverse-transcription polymerase chain reaction (RT-PCR) for the expression of MAGE-1, MAGE-3, BAGE and GAGE1-2. Endometrial carcinoma cell were treated with 5-aza-2’-deoxycytidine (DAC) in co-culture, the growth curves were observed; the genes expression pattern before and after drug treatment was measured by RT-PCR.Results: In 35 endometrial carcinoma of samples, MAGE-1, MAGE-3, BAGE and GAGE1 -2 mRNAs were expressed in 46% (16/35), 49% (17/35), 20% (7/35) and 26% (9/35) , respectively. At least one transcript was detected in 77%. While no expression was observed in 15 normal endometrial tissues. There was no significant更多还原