【作者】 刘洪元；

【导师】 徐贵发；

【作者基本信息】 山东大学 ， 公共卫生， 2006， 硕士

【摘要】 研究目的：了解鲜马奶的抗疲劳作用。 研究方法： 1、采用化学分析方法对马奶中相关的抗疲劳营养素的含量进行测定； 2、给小鼠喂饲不同剂量的马奶，以昆明种成年雄性小鼠为实验对象，设立一个对照组和高、中、低（21ml/kg．d、42ml/kg．d、84ml/kg．d）三个实验剂量组，喂饲35天后，观察小鼠负重游泳时间和爬竿时间，喂饲40天后，测定小鼠运动后血乳酸、血乳酸脱氢酶活性、肝糖原及尿素氮含量。 结果： 1、马奶中含乳糖7.83％；蛋白质2.82％，支链氨基酸20.2mg/100ml，占总氨基酸含量的20％，牛磺酸13.27mg/100ml；马奶中含有Ca、P、Fe、Se、Zn、I、Cu等无机盐；马奶中含有维生素A、维生素E、维生素B₁、维生素B₂、尼克酸、维生素C等维生素，其中维生素C含量达到33.53mg/100ml。 2、在负重游泳实验中，小鼠负重游泳时间，高剂量组与对照组相比时间延长，差别有显著意义（P＜0.05）。 3、高、中、低剂量组爬杆持续时间均明显比对照组延长，差别有非常显著意义（P＜0.01）。 4、小鼠的运动后血乳酸含量，高、中、低剂量组均明显低于对照组（P＜0.01）。 5、小鼠运动后血乳酸脱氢酶活力，中、高剂量组明显高于对照组（P＜0.05，P＜0.01）； 6、小鼠运动后，高剂量组肝糖原储备量高于对照组（P＜0.05）。 7、小鼠运动后，中、高剂量组血清尿素氮含量与对照组相比明显下降，有非常显著性差异（P＜0.01，P＜0.01）。更多还原

【Abstract】 Objective: To study the anti-fatigue effect of fresh Mare’s milk.Methods:1. The chemical methods were used to analyze the anti-fatigue nutrients content in Mare’s milk2. The mice were randomly devided into 4 groups: three of which were experimental groups feeding with Mare’s milk at doses of 21,42, 84ml/(kg.d) , and the rest was control group. After 35 days, Load swimming time and lasting time of climbing pole in mice were measured. After 40 days, blood lactic acid, blood lactate dehydrogenises activity, liver glycogen, and serum urea nitrogen were measured.Results:1. Mare’s milk contains lactose 7.83%; protein 2.82%, branch- chain amino acid 20.2mg/100ml (20% of the total protein content), taurine 13.27mg/100ml; Mare’s milk contains Ca, P, Fe, Se, Zn, I, Cu and vitamin A, vitamin E, vitamin B1, vitamin B2, calcium, vitamin C (33.53mg/100ml).2.It showed that there was significantly difference the in Load swimming time between the high dose and control groups after 35 days’ administration (P < 0.05).3. Three Mare’s milk groups showed significantly decrease of lasting time of climbing pole in mice after 35 days’ administration (P < 0.01).4. Three Mare’s milk groups showed decrease of blood lactic acid in swimming mice after 40 days’ administration (P < 0.01).5. The medium and high dose of mare’s milk groups showed increase of the blood lactate dehydrogenises activity in swimming mice after 40 days’ administration更多还原