杜泊绵羊冷冻胚胎移植研究

【作者】 孙洁；

【导师】 王公金； 李朝军；

【作者基本信息】 南京师范大学 ， 发育生物学， 2006， 硕士

【摘要】 本实验对从新西兰引进的杜泊绵羊冷冻胚胎进行了移植研究，通过设置不同季节、不同受体等条件对杜泊绵羊冷冻胚胎移植效果进行研究。研究结果如下：受体羊总平均同期发情率达72.56％，胚胎移植利用率达49.66％，妊娠率达57.05％，移植胚胎的产羔率达53.86％，羔羊出生重为4.33kg(♂)和4.27kg(♀)，羔羊1月龄、3月龄与6月龄的平均体重分别达14.45kg、29.58kg和46.54kg；4批次实验共移植冷冻胚胎440枚，产羔251只，现存活231只。研究结果分析表明：不同批次和不同品种受体间胚移效果存在不同程度的差异；秋、冬季进行胚胎移植效果较春季好；湖羊作为杜泊绵羊冷冻胚胎移植受体比小尾寒羊更佳。 实验最终表明：在秋、冬季对杜泊绵羊冷冻胚胎以湖羊作为受体进行移植，可以获得更好的胚胎移植效果。本研究可作为今后杜泊绵羊繁育研究的参考依据，可望在畜牧业科研和生产中广泛推广应用。更多还原

【Abstract】 This paper investigated that the cryopreserved embryos of Dorper sheep imported from New Zealand were transferred into the recipients of Hu sheep and Han sheep in different seasons, and the efficiency of the frozen embryo transfer was analyzed and estimated. The results showed that the average of estrus synchronization ratio of total recipients was 72.56%, utilization ratio of lambs in estrus was 49.66%, pregnancy ratio was 57.05%, birth rate was 53.86%, birth weights of lambs were 4.33 kg (in male) and 4.27 kg (in female), and lamb weights of one month, three months and six months were 14.45 kg, 29.58 kg, and 46.54 kg respectively. 440 frozen embryos in total were transferred into 440 recipients in the 4-batch experiments, in which 251 lambs were born, and 231 lambs survived. There were also different results of embryo transfer experiments within batches or breeds of recipients. The efficiency of embryo transfer in Autumn and Winter is higher than in Spring. Hu Sheep is better than Han Sheep in each batch.It is concluded that the cryopreserved embryos of Dorper sheep transferred into the recipients of Hu Sheep in Autumn and Winter is more reliable and more effective. This result is expected to be applied widely to researcher and reproduction of animal husbandry field.更多还原