【作者】 皮艳俐；

【导师】 马季骅；

【作者基本信息】 武汉科技大学 ， 心脏电生理， 2006， 硕士

【摘要】 目的:研究中华大蟾蜍卵母细胞内源性电压门控型离子通道的成份及其生理特性。方法:采用双微电极电压钳法。结果:(1)发现卵母细胞去极化至-30mv及更偏正时,有一持续的电压依赖性的外向电流出现。该电流被最大抑制剂量的钾离子通道拮抗剂TEA(10mmol/L)和4-AP(10mmol/L)协同作用时,只能被抑制到最大电流幅度的23.4±2.4%;(2)用无氯Ringer氏液与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时,可将此电流基本抑制,仅剩2.2±0.2%。用氯离子通道拮抗剂NPPB(30μmol/L)与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时,该电流也可被基本抑制,只剩2.1±0.2%;(3)用无钙Ringer氏液与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时,可将此电流基本抑制,仅剩3.2±0.3%。用钙离子通道拮抗剂维拉帕米(40μmol/L),与钾离子通道拮抗剂(TEA10mmol/L和4-AP10mmol/L)协同作用时,该电流也可被基本抑制,只剩3.1±0.3%。结论:中华大蟾蜍卵母细胞质膜上除有钾离子通道之外,还存在钙依赖性的氯离子通道.更多还原

【Abstract】 Objective: To study membrane properties of the fully-grown oocytes from toad, Bufo bufo gargarizans.Method: Two-microelectrode voltage-clamp(TEA)method was used.Results: (1)It was found that a sustained outward current was elicited by membrane depolarization to -30mv or more positive value. Potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) partly reduced the majority of outward current to 23.4±2.4%.(2)Bath application of Cl—-free Ringer’s solution and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 2.2±0.2% .Bath application of chloride channel blocke(rNPPB30μmol/L)and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 2.1±0.2%.Bath application of Ca2+-free Ringer’s solution and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remaining 3.2±0.3%.(3) Bath application of calcium channel blockers(Verapamil40μmol/L)and potassium channel blockers(TEA10mmol/L and 4-AP10mmol/L) could almost completely block the outward current, only remain 3.1±0.3%.Conclusion: It was firstly concluded that plasma membrane of Bufo oocyte exist calcium-dependent chloride channel, except potassium channel.更多还原