动物营养液中维生素的分析方法研究

【作者】 郝荣辉；

【导师】 谷学新；

【作者基本信息】 首都师范大学 ， 分析化学， 2006， 硕士

【摘要】 维生素是维持机体正常生长必不可缺的化合物，动物胺基维他营养液是一种新型含有丰富维生素以及氨基酸的新型动物饲料添加剂，可以提高动物机体的免疫力。 本文第一部分介绍了动物营养液中各维生素的结构、生理功能以及维生素缺乏与过量对肌体的影响。综述了脂溶性维生素VA、VD、VE测定方法的研究进展，水溶性B族维生素测定方法的研究进展以及分光光度法分析VC与VK₃的研究进展。 第二部分系统地建立了动物营养液中各维生素的分析方法。建立了反相高效液相色谱法二极管阵列检测器三波长下同时测定动物营养液中脂溶性维生素VA、VD₃、VE的方法，采用ZORBAX SB-C₁₈柱（2.1mm×150mm，5μm），以异丙醇-水（65：35）为流动相，流速0.3 mL／min，在波长325nm，265 nm和290nm三波长下测定；建立了反相高效液相色谱法同时测定动物营养液中四种B族维生素的方法，采用AichromBond-AQ C₁₈（4.6mm×250mm，5μm）柱，以甲醇-缓冲盐溶液为流动相梯度洗脱，二极管阵列检测器三波长下测定；以及建立了分光光度法分别测定动物营养液中VC与VK₃分析方法。不同的检测对象选择了不同的分析方法，方法灵活，结果满意，可有效的控制动物营养液产品的质量。 第三部分综述了果蔬农药残留速测技术以及酶联免疫吸附测定技术在食品检验中的应用，建立了用酶联免疫技术快速测定蔬菜和水果中农残的分析方法。此方法专一性强、灵敏度高、抗干扰性强、测试速度快并且操作简单。更多还原

【Abstract】 Vitamin is an indispensable compound to maintain normal growth of organisms. Nutrient solution for animals is a new animal feed additive, which is containing vitamin and nutrient rich. It can enhance the immunity of animal organisms.The structure, physiological functions of vitamins in nutrient solution for animals and the impact on organism of vitamins’ deficiencies and intake of excessive vitamins were introduced in the first part of this thesis. The progress of analytical methods of fat-soluble vitamin A, D, E and the progress of analytical methods of vitamins B were summarized in this thesis. In addition, the progresses of analytical methods of vitamin C and K₃ by spectrophotometry were reviewed.The analytical methods of vitamins in nutrient solution for animals were established systematically in the second part of this paper. A method for the simultaneous determination of fat-soluble vitamin A, D₃ and E in nutrient solution for animals by HPLC-diode-array detector was developed. A ZORBAX SB-C₁₈ column （2.1 mm×150 mm. 5 μm） was selected as separation. The mobile phase was isopropanol-water （65:35） . The detection wavelength of vitamin A, D₃ and E were at 325 nm, 265 nm and 290 nm, respectively. A reversed phase high performance liquid chromatographic method for the simultaneous determination of vitamins B in nutrient solution for animals was developed. An AichromBond-AQ C₁₈ column （4.6 mm × 250 mm, 5 μm） was selected as separation. The mobile phase was methanol / potassium dihydrogen phosphate buffer. The detector was HPLC-diode-array detector and The detection wavelengths were at three wavelengths, respectively. In addition, the analytical methods of vitamin C and K3 by spectrophotometry were developed, respectively.Different objects chose different analytical methods. The methods were flexible and the results were satisfactory, which could control the quality of the products effectively.The technique of rapid testing pesticide residue in fruits and vegetables and the applications of ELISA in the field of food analysis were reviewed in the third part. A method for the determination of pesticide residues in vegetables and fruits by ELISA was developed. This method was very specific, with high anti-interfering ability. It was also highly sensitive,time-saving and easy to operate.更多还原