【作者】 罗国良；

【导师】 李文平； 马美湖；

【作者基本信息】 湖南农业大学 ， 特种经济动物饲养， 2006， 硕士

【摘要】 【目的】 本研究根据相关的研究和现代中医药理论以及我们所做的预备试验精心设计茶多酚复方制剂。通过试验主要研究茶多酚复方制剂对S180肿瘤的体内、外抑制效果和诱导肿瘤细胞凋亡的机理以及与抑瘤作用相关的血液生化指标及免疫力指标。 【方法】 体外试验采用细胞培养，MTT法检测不同浓度和作用时间的茶多酚复方制剂对S180细胞的抑瘤效果。体内试验采用可移植肿瘤试验法，观察茶多酚复方制剂的抑瘤效果；检测肿瘤相关血清酶(过氧化氢酶，乳酸脱氢酶，谷胱甘肽过氧化物酶)活力，测定免疫器官指数；从组织形态学，DNA梯状条带，免疫组化观察bcl-2表达三个方面观察茶多酚复方制剂诱导肿瘤细胞凋亡的作用。 【结果】 体外MTT试验结果：茶多酚复方制剂低、中、高剂量作用48和72小时的抑瘤率分别为：35.92％、42.88％、53.85％；41.29％、46.58％、61.63％；体内试验结果表明，低、高剂量预防组，低、高剂量治疗组抑瘤率分别为：30.63％，37.91％，32.60％，37.95％；不同处理的药物组的胸腺指数与脾脏指数均有明显的提高；各试验组的LDH活力显著降低，CAT、6SH-PX活力显著升高；各试验组肿瘤中可见凋亡细胞，DNA梯状条带明显，bcl-2表达下降。在相同的茶多酚剂量下，预防处理组的抑瘤效果要明显优于未经预防处理的抑瘤效果。 【结论】 茶多酚复方制剂在一定剂量范围有明显抑瘤效果，且抑瘤率与剂量和时间呈正相关；该复方制剂对肿瘤有一定的预防作用；茶多酚复方制剂抑瘤的机理为提高机体免疫力，改变与肿瘤代谢相关的酶活力，诱导肿瘤细胞凋亡。更多还原

【Abstract】 [Objective] The experiment designed tea polyphenols compound (TPC) according to the related studies, theories of modern Chinese medicine and the prepared trials we did. Mainly to study the anticancer effect of tea polyphenols compound (TPC) in vivo and in vitro and the mechanism of inducing apoptosis and blood biochemical Parameters related to anticancer effects as well as the Indexes of immune organ in Tumor-bearing Mice.[Methods] The tea polyphenols compound (TPC) was used to detect the anticancer effects on S180 cell line by means of MTT in vitro. By means of transplantable tumor in vivo, to observe the inhibitory effect of TPC on S180 cells proliferation; assay the serum enzymes activity related to tumor (Catalase, Lactate dehydrogenase, Glutathione peroxidase); measure indexes of immune organs; observe the effects of tea polyphenols compound (TPC) inducing apoptosis by histology and morphology, the DNA ladder and the expression of Bcl-2 were examined.[Results] The results in vitro showed that 100 mg/L, 200 mg/L, 400 mg/L tea polyphenols compound (TPC) had significantly anticancer effects on S180 cell for 48 hours with the antineoplastic rate of 35.92%, 42.88%, 53.85%, respectively, while for 72 hours with the antineoplastic rate of 41.29%, 46.58%, 61.63%, respectively. The results in vivo showed that the antineoplastic rates of low dose inhibitory group, high dose inhibitory group, low dose therapy group and high dose therapy group were 30.63%, 37.91%, 32.60%, 37.95%, respectively; Compared with the control group, with TCP drenched, the indexes of thymus and spleen among different experimental groups had significantly increased; with TCP drenched, LDH activity among experimental groups had significantly decreased, while activities of CAT and GSH-PX had significantly increased. Typical apoptotic changes were observed in tumor in experimental groups under the microscope. Compare with the control group, the experimental groups had also obvious DNA ladder and lower levels of Bcl-2 expression. Under the same dose of TPC, the anticancer effects of preventable groups更多还原