【作者】 田燕；

【导师】 王洁；

【作者基本信息】 河北医科大学 ， 口腔基础医学， 2006， 硕士

【摘要】 目的:涎腺腺样囊性癌(adenoid cystic carcinoma,ACC),是一种常见的涎腺恶性肿瘤。其恶性程度高,侵袭性强,易复发、易早期转移。且对放疗、化疗不敏感,手术治疗难以根除。因此,寻求有效的辅助性治疗手段非常必要。肿瘤基因治疗已成为最引人瞩目的研究领域,是继手术、放疗、化疗、免疫治疗之后的第5种治疗模式。而自杀基因疗法是目前有效治疗肿瘤中最有前景的基因治疗策略之一。本研究目的在于探讨腺病毒载体介导的单纯疱疹病毒胸苷激酶(HSV-tk)与免疫因子白细胞介素-2(IL-2)联合应用对涎腺腺样囊性癌的抑制和杀伤作用。可望为该肿瘤基因治疗的临床研究提供可靠的理论依据和实验室数据。材料与方法:1材料(1)实验材料:人涎腺腺样囊性癌高转移细胞系(ACC-M)。(2)药品:腺病毒介导的单纯疱疹病毒胸苷激酶(HSV-tk),腺病毒介导白细胞介素-2(IL-2),丙氧鸟苷(GCV)(3)实验动物:20只SPF级雌性Balb/c裸小鼠,4~5周龄。2方法:(1)动物模型建立:ACC-M细胞培养于RPMI1640含胎牛血清和双抗的培养液中,37℃恒温孵育,收集细胞并调整为2×10~7/ml的细胞悬液。将ACC-M细胞悬液接种于裸鼠皮下,每只注射左前背部和右后背部2点,每点0.2ml,待移植瘤直径达约1.0cm,随机分组。(2)分组与给药:共分4组,每组5只。均采取瘤体内注射。A组空白对照组,每移植瘤注射与实验组等量生理盐水。B组更多还原

【Abstract】 Objective: Salivary adenoid cystic carcinoma (SACC) is a very common malignancy of salivary gland. Recurrence and early metastasis are its biological properties. It is difficult to be entirely resected surgically and is invalid to radiotherapy and chemotherapy. So the long-term prognosis of patients is very dismal. Therefore, it is imperative to find a perfect auxiliary therapeutic treatment. As a new method for tumor therapy, gene therapy has a large development. Herpes simplex virus thymidine kinase/ganciclovir (HSV-TK/GCV) method is one of the most frequently utilized measures of gene therapy. However, suicide gene therapy alone cannot completely eliminate tumor cells, so it is necessary to combine suicide gene therapy with other therapy to improve performance. This study is to observe the depressing kill effect on SACC animal model in nude mice by union treated with HSV-TK and IL-2 adenovirus vector.Materials and Methods: 1 Materials: (1) Cell line:a human adenoid cystic carcinoma cell line with high tendency of lung metastasi(sACC-M); (2) Drug: adenovirus vector HSV-TK, adenovirus vector IL-2, ganciclovir (GCV); (3) Animal: Twenty SPF grade, 4~6 week-old female Balb/c nude mice were used in this study. 2 Methods: (1) animal model: ACC-M cells were更多还原