【作者】 林武；

【导师】 赵铖；

【作者基本信息】 广西医科大学 ， 内科学， 2006， 硕士

【摘要】 目的:探讨广西地区汉族系统性红斑狼疮( Systemic lupus erythematosus, SLE)患者与HLA-DQA1等位基因的相关性。方法:用聚合酶链式反应-序列特异性引物(PCR-SSP)法对64例广西汉族SLE患者及60例汉族健康人群的HLA-DQA1频率进行检测。结果:1.用12对DQA1引物检出HLA-DQA1*0101、0102、0103、0104、0201、0301、0302、0401、0501、0601共10个亚型;SLE组HLA-DQA1*0101、0102等位基因频率较正常对照组显著升高(χ2 =8.627 , P=0.003,RR=3.421及χ2=8.965,P=0.003,RR=3.314);狼疮性肾炎(Lupus nephritis,LN)组HLA-DQA1*0102等位基因频率较无LN表现的SLE组显著升高(χ2 =6.418 , P=0.011,RR=4.688);LN组HLA-DQA1*0501等位基因频率较无LN表现的SLE组显著降低(χ2 =6.130 , P=0.013,RR=0.172);未发现与LN病理类型显著相关的HLA-DQA1等位基因。结论:DQA1*0101、0102可能是广西汉族SLE的易感基因;DQA1*0101可能是广西汉族LN的易感基因,HLA-DQA1*0501可能是广西汉族LN的保护基因;未发现与LN病理类型显著相关的HLA-DQA1等位基因。更多还原

【Abstract】 Objective: To explore the association of HLA-DQA1 alleles with Systemic lupus erythematosus of Han nationality in Guangxi.Methods:Polymerase chain reaction-sequence specific primers technique(PCR-SSP)was used to comparatively study the allelic genes in HLA-DQA1 locus in 64 patients with SLE and 60 selected unrelated healthy controls of Han subjects.Results: Twelve pairs primers amplified out 10 isoforms within HLA-DQA1 region:HLA-DQA1*0101、0102、0103、0104、0201、0301、0302、0401、0501、0601。The allelic frequencies of HLA-DQA1*0101、0102 was significantly increased in SLE patients than those of the controls(χ2=8.627,P=0.003 ,RR=3.421;χ2=8.965,P=0.003 ,RR=3.314); The allelic frequencies of HLA-DQA1*0102 was significantly increased in LN patients than those of the non-LN SLE patients(χ2 =6.418 , P=0.011, RR=4.688),but the allelic frequencies of HLA-DQA1*0501 was significantly decreased in LN patients than those of the non-LN SLE patients(χ2 =6.130 , P=0.013,RR=0.172);We have no found the association of HLA-DQA1 alleles with the renal pathologic of LN.Conclusion:The HLA-DQA1*0101、0102 allele may be susceptible gene for SLE in Guangxi Han nationality.The HLA-DQA1*0101 may be susceptible gene for LN in Guangxi Han Nationality. The HLA-DQA1*0501 may be protecting gene for LN in Guangxi Han Nationality. We have no found the association of HLA-DQA1 alleles with the pathologic of LN.更多还原