【作者】 韩克杰；

【导师】 邢世岩；

【作者基本信息】 山东农业大学 ， 森林培育， 2006， 硕士

【摘要】 对欧洲榛子(Coryus avellana L.)种仁采收前后、采后贮藏期间、萌发后以及PEG、NAA、IBA、2,4-D处理下的生理生化指标的测定分析结果表明:1.采收前后的实验结果表明:采收后种仁含水量、淀粉含量比采收前低,粗脂肪含量、可溶性糖含量、游离氨基酸总量,可溶性蛋白的含量、SOD、CAT、POD活性均高于采收前,采收前后粗脂肪含量稳定的品种是1#、2#、3#、4#、7#。采收前的种仁含水量,可溶性蛋糖,淀粉含量高于平榛(Corylus heterphylla F.)而粗脂肪含量、游离氨基酸总量、可溶性蛋白含量、淀粉酶活性、SOD、CAT、POD活性低于平榛,采收后的游离氨基酸总量、β-淀粉酶活性高于平榛而含水量、粗脂肪含量、可溶性糖含量、淀粉含量、可溶性蛋白含量、α-淀粉酶活性、POD、CAT、SOD活性均低于平榛;欧洲榛子9个品种间采收前含水量、粗脂肪含量、可溶性糖含量略有差异,而游离氨基酸、可溶性蛋白含量、淀粉酶活性、POD、CAT、SOD活性各品种间差异均极显著,采收后品种间可溶性糖、淀粉含量差异显著,而可溶性蛋白、游离氨基酸总量、SOD、CAT、POD、淀粉酶活性差异极显著,聚类分结果表明,采收前1#、3#、7#品种是高脂、高蛋白品种,4#是高碳水化合物品种,采收后7#品种为高脂高淀粉品种,8#为高蛋白和高糖品种。2.不同方法处理种子的实验表明,可容性蛋白、游离氨基酸总量、CAT活性、POD活性均是沙藏种子大于干藏的种子且差异显著,粗脂肪含量和淀粉含量无显著差异;不同PEG浓度处理种子的实验结果表明,在10%以下的浓度,随PEG浓度的递增,可溶性蛋白、SOD活性和CAT活性升高,该浓度以上,三者变小,说明10%的PEG处理是最佳浓度;综合处理的试验表明可溶性蛋白的最佳处理组合是10%PEG+沙藏+NAA,α-淀粉酶和β-淀粉酶的最佳组合分别是10%PEG+沙藏+IBA和10%PEG+冷干藏+NAA,而20% PEG+沙藏+NAA、30% PEG+沙藏+2,4-D、10% PEG+沙藏+NAA则分别是SOD、CAT、POD的最佳处理。3.不同贮藏时间动态研究证明在采后一年的贮藏期内,粗脂肪含量更多还原

【Abstract】 The physiological and biochemical index of Corylus avellana L. kernel was studied during harvest, storage, germination and after PEG、NAA、2,4-D treatment .The results were as follows:1.The water and starch content of kernel after harvest were lower than that before harvest. The crude fat, soluble protein, free amino acid, soluble sugar content and vigor of SOD、CAT、POD were higher than that before harvest. The cultivars with steady crude fat content of kernel were 1#、2#、3#、4#and 7#. Before reap, the water content, soluble sugar, starch content of kernel before harvest were higher than Corlus heherphylla F. , while the crude fat, free amino acid, soluble protein content and vigor of amylase, SOD, CAT and POD were lower than Corlus heherphylla F. . After harvest, the free amino acid andβ-Amylase vigor were higher and the water、crude fat, soluble sugar, soluble protein content and vigor ofα-Amylase, SOD, CAT, POD were lower than Corlus heherphylla F. . Before harvest, the content of water, crude fat, soluble sugar were slightly different among each Corylus avellana L. cultivar. However, there was extremely significant difference among cultivars with regard to free amino acid, soluble protein content and amylase, SOD, CAT, POD vigor. After harvest ,the difference of soluble sugar and starch content were significant and difference of soluble protein free amino acid content and vigor of SOD,CAT, POD, amylase were extremely significant. The results of cluster analysis on fat, sugar, starch, protein, amino acid to various hazelnut cultivars showed that 1#、3#、7# and 4# was cultivar that had high content of crude fat and soluble protein and high carbohydrate content before harvest and更多还原