【作者】 张金波；

【导师】 吕作舟；

【作者基本信息】 华中农业大学 ， 微生物学， 2005， 硕士

【摘要】 本研究主要包括阿魏侧耳液体培养基的筛选、发酵条件的优化和胞外多糖理化性质的分析三方面的内容,其结果如下: 1、通过单因子试验和正交试验,确定适合菌丝生长的优化培养基配方为:VBl0.002%,葡萄糖3%,蛋白胨0.15%,（NH4）₂SO₄0.05%,酵母膏0.3,MgSO₄0.05%,KH₂PO₄0.1%,ZnSO₄0.02%,NaCl0.03%。适合产生胞外多糖的优化培养基配方为:VBl0.002%,葡萄糖1%,蛋白胨0.15%,（NH4）₂SO₄0.05%,酵母膏0.3,MgSO₄0.05%,KH₂PO₄0.05%,ZnSO₄0.01%,NaCl0.03%。 2、确定摇瓶（250mL）发酵条件为:装液量100mL,接种量10%,起始pH5.0,培养温度25℃,摇床转速150r/min,接种菌龄5d,发酵10d。50L发酵罐发酵条件为装液量30L,接种菌龄5d,接种量10%,温度25℃,起始pH5.0,转速150r/min,通气量1.2m³/h,罐压0.04Mpa,培养108h。 3、DEAE-纤维素柱层析结果表明:胞外水溶性多糖含有一个中性多糖组分和一个糖蛋白组分。 4、气相色谱分析结果表明:胞外水不溶性多糖由鼠李糖、葡萄糖和4种未知单糖组成,鼠李糖和葡萄糖的摩尔比为:鼠李糖:葡萄糖=4.08:5.55更多还原

【Abstract】 This paper included mainly three aspects: screening of liquid culture medium of Pleurotus ferulae Lanzi, optimization of fermentation conditions, analyses of physical and chemical properties of EPS, the results of which were as follows:1. By monofactorial and orthogonal test, the optimal composition of liquid culture medium for the mycelia’s growth and the producing of EPS were respectively determined. The former was composed by 0.002% VB₁, 3% Glucose, 0. 15% Peptone, 0. 05% （NH4）₂SO₄, 0.3% extract of yeast, 0.05% MgSO₄, 0. 1%KH₂PO₄,0.02%ZnSO₄ , 0. 03%NaCl. The later was composed by 0.002% VB₁, 1% Glucose, 0. 15% Peptone, 0.05% （NH4）₂SO₄, 0.3% extract of yeast, 0.05% MgSO₄, 0. 05%KH₂PO₄, 0. 01%ZnSO₄, 0. 03%NaCl.2. The optimal flask （250ml） fermentation conditions were as follows: culture volume was 100mL, inoculum quantity was 10%, initial pH value was 5.0, culture temperature was 25℃, rotation speed was 150rpm, strain age of spawn was 5 days, culture time was 10 days.The optimal jar （50L） fermentation conditions were as follows: culture volume was 30L, strain age of spawn was 5 days, inoculum quantity was 10%, initial pH value was 5.0, culture temperature was 25 ℃, rotation speed was 150rpm, ventilation quantity was 1. 2m³/h , pressure inside the jar was 0. 04Mpa , culture time was 108 hours.3. The result of DEAE-cellulose elution indicated that there was one neutral polysaccharide and one glycoprotein in the water-soluble EPS.4. Analysed with gas chromatography the monosaccharide composition and molecular ratio of water-fast EPS was gained: rha:glu = 4. 08:5. 55, and 4 unknownmonosaccharide.更多还原