【作者】 柳华；

【导师】 彭芝兰；

【作者基本信息】 四川大学 ， 妇产科学， 2005， 硕士

【摘要】 研究目的:宫颈癌是在全球妇女恶性肿瘤中发病率仅次于乳腺癌的最常见的恶性肿瘤,每年约有20多万妇女死于宫颈癌。而宫颈间质内富含Ⅳ型胶原,层粘连蛋白,纤维结合素及氨基葡聚糖等,多为MMP-7的降解底物,TIMP-1则对MMP-7有抑制作用,二者及其之间的平衡在宫颈癌的发生发展中作用重要。故本试验拟通过检测宫颈癌中MMP-7,TIMP-1及二者之间平衡关系的表达情况,来探寻其与宫颈癌的侵袭转移及预后的关系,以寻求新的预后判定指标及新的治疗途径。 研究方法:本研究采用免疫组化SP法对53例宫颈浸润癌组织,9例CIN,10例因子宫肌瘤而行子宫切除的正常宫颈组织标本进行MMP-7及TIMP-1蛋白表达的检测。按显色的强弱及阳性细胞所占的比例分级。 研究结果:1.MMP-7,TIMP-1在宫颈癌中的表达均高于CIN及正常宫颈组织(p<0.01),其蛋白主要表达于癌细胞的胞浆中。 2.MMP-7与TIMP-1呈弱的正相关关系(r=0.281,p<0.05),其中MMP-7在宫颈癌中多为强阳性表达,而TIMP-1则多为弱阳性表达。 3.MMP-7在宫颈鳞癌中的表达高于腺癌,低分化癌中的表达高于中-高分化者,伴淋巴结转移者中的表达高于无淋巴结转移者(p<0.05),而与患者的年龄,临床分期则无明显相关关系(p>0.05)。TIMP-1与宫颈癌更多还原

【Abstract】 Objective: cervical cancinoma is the second most familiar malignancy following mammary carcinoma among women all over the world, and about 200,000 women die of it annually. As is known to all, cervix is consisted of IV collagen, laminin, fibrin combine element and glycosaminoglycans, which are mostly the substrate of matrix metalloproteinase-7, and tissue inhibitor of matrix metalloproteinase-1 is its’ inhibitor. Both MMP-7, TIMP-1 and the balance between them play an important role in the arising and expanding of cervical carcinoma. The aim of this research is to investigate the expression of MMP-7 and TIMP-1 in the cervical carcinoma and to evaluate the relationship between the expression of both MMP-7, TIMP-1 and clinicpathologic features of cervical carcinoma in order to seek new prognosis index, and new therapy approach.Methods: The expression and distribution of MMP-7 and TIMP-1 proteins are detected by immunohistochemical technique in 53 cases of cervical carcinoma tissue, 9 cases of CIN and 10 cases of normal cervices tissue coming from operation specimen excised because of myoma of uterus.Classification is done according to the depth of the color and the proportion of the masculine cell.Results: l.The level of MMP-7 and TIMP-1 are significantly higher in cervical carcinoma than in CIN and normal cervices (p<0.01). The protein mainly expresses in the cytoplasm of cervical carcinoma cells. 2. There is a weak relationship between MMP-7 and TIMP-1 (r=0.281, p<0.05). And the expression of MMP-7 is strong in most cases while that of TIMP-1 is weak in most ones.3.The expression of MMP-7 is related to pathological classification, differentiation and lymph node metastasis (p<0.05), and has nothing to do with age, clinical stages (p>0.05). However there is no sign showing the expression of TIMP-1 has something to do with the biological behavior of cervical carcinoma in carcinoma cell (p>0.05).Conclusion: MMP-7 owns its importance in the invasion, metastasis and prognosis of cervical carcinoma, which can act as the new therapy target and be inhibited by TIMP-1.更多还原