【作者】 李家兵；

【导师】 唐伟；

【作者基本信息】 重庆医科大学 ， 外科学， 2005， 硕士

【摘要】 目的:研究细胞周期蛋白依赖性激酶抑制因子 p57kip2、增殖细胞核抗原(PCNA)在膀胱移行细胞癌(Bladder transitional cell carcinoma,BTCC)中的表达及其相关性,以探讨其在膀胱癌的发生、发展中所起的作用及预后的关系。 方法:选取重庆医科大学附属第一医院泌尿外科 1994—1997年 膀 胱 移 行 细 胞 癌 标 本 50 例 。 运 用 免 疫 组 织 化 学 方 法(EliVisionTMplus 二步法)检测标本中 p57kip2、PCNA 的表达情况,综合分析两者在膀胱移行细胞癌中的表达情况及其相关性。 结果:p57kip2和 PCNA 在本组研究的 50 例标本中均有不同程度的表达。p57kip2 阳性表达率在膀胱移行细胞癌组织中为46%(23/50),显著低于正常膀胱粘膜组织 78.57% (11/14()p<0.05),并与膀胱移行细胞癌组织病理分级﹑生存率显著相关(p<0.05),但与临床分期无关(p>0.05)。PCNA 阳性表达率在膀胱移行细胞癌组织中为 60%(30/50),显著高于正常膀胱粘膜组织的 0%(0/14) (p<0.01),并与膀胱移行细胞癌组织病理分级,临床分期和生存率显著相关(p<0.05)。p57kip2和 PCNA 的表达呈负相关(p<0.01)。 结论:1.p57kip2低表达和 PCNA 过度表达,可能参与膀胱肿瘤的诱发。 2.p57kip2 与膀胱肿瘤的病理分级、患者生存率均有显著相关,但与临床分期无关,提示 p57kip2可反映膀胱移行细胞癌细胞分化状态及恶性程度的一种指标,而与肿瘤浸润深度无明显关系。p57kip2低表达可以作为不良预后的一个参考指标。PCNA 与膀胱肿瘤的病更多还原

【Abstract】 Objective: TO study expressions and correlations of p57kip2、PCNA in bladder transitional cell carcinoma(BTCC)and discuss their significance in carcinogenesis and progression of BTCC cancer,as well as their relations with angiogenesis and prognosis. Methods: Fifty specimens of BTCC between 1994 and 1997 at the department of Urology,the First Affiliated Hospital Chongqing Medical University,in the study group.The expressions of p57kip2 and PCNA were tested by immunohistochemical method(EliVisionTMplus). The results were reciprocally analyzed and compared with angiogenesis and prognosis. Results: p57kip2 and PCNA positive stained were a greater or lesser degree detected in all of the 50 cases.Positive rate of p57kip2 in BTCC was 46%,which was significant lower than 78.57% in normal bladder muscosa.(χ2=4.660,P <0.05),p57kip2 expression correlated remarkably with both grade and survival(χ2=8.592,P<0.05;χ2=4.546,P<0.05), but did not correlate clinical stage(χ2=0.573,P>0.05);positive rate of PCNA in BTCC was 60 %(30/50),which was significant higher than 0%(0/14)in normal bladder muscosa(χ2=15.812, p <0.01),PCNA expression correlated remarkably with both grade ,stage and survival(P<0.05)there was a highly significant inverse correlation between the expression of p57kip2 and PCNA(r=-0.443,P <0.01) Conclusion: l. The low express of p57kip2 and overexpression of PCNA are closely related to tumorigenesis of bladder cancer. 2. p57kip2 expression correlated remarkably with both grade and survival,but did not correlate clinical stage,indicating that p57kip2 is related to the differentiation and malignant degree of bladder cancer,not related to tumor infiltration,it has a poor prognosis.PCNA expression correlated remarkably with both grade ,stage and survival, indicating PCNA are related to the differentiation and progression of bladder cancer and it might be useful prognosistic markers in bladder carcinoma. 3.There was a negative correlation between the expression rate of p57kip2 and PCNA in bladder cancer, indicating that they have synergism in the happening and development of bladder cancer.At the same time they may have some feedback loop in progression of bladder cancer.更多还原