【作者】 田加坤；

【导师】 霍钢；

【作者基本信息】 重庆医科大学 ， 神经外科， 2005， 硕士

【摘要】 目的:从基因水平对 MMP-2、TIMP-2 及 CD147 在垂体腺瘤中的表达作出进一步研究。结合第一部分结果,了解它们在基因和蛋白质水平中的表达是否具有一致性,探讨它们在垂体腺瘤侵袭性中的作用机制。 方法:将临床资料分为侵袭性腺瘤组和非侵袭性腺瘤组,应用逆转录 PCR(RT-PCR)对 55 例垂体腺瘤患者标本 MMP-2、TIMP-2 及CD147 的 mRNA 表达进行检测。分析该三项指标表达与肿瘤侵袭性的关系以及 CD147、TIMP-2 mRNA 与 MMP-2 mRNA 表达的相关性。 结果:55 例垂体瘤患者中,32 例为侵袭性垂体腺瘤,23 例为非侵袭性垂体腺瘤。侵袭性垂体腺瘤组的 CD147 与 MMP-2 mRNA 表达均较非侵袭性腺瘤组增高,而 TIMP-2 mRNA 表达则下降,差异均具有统计学意义(P <0.01)。侵袭性腺瘤组中 MMP-2 与 CD147 mRNA 的表达成正相关(r=0.73,P<0.05), 非侵袭性腺瘤组中 MMP-2 与 CD147 的表达无相关性(r=0.34,P>0.05)。侵袭性腺瘤组中 MMP-2 与 TIMP-2 mRNA表达呈负相关(r=-0.67,P<0.05),而非侵袭性腺瘤组中无相关性(r=-0.24, P>0.05)。 结论:垂体腺瘤的侵袭性与 CD147 与 MMP-2 mRNA 高表达及TIMP-2 mRNA 低表达密切相关,在肿瘤侵袭过程中 CD147 与 MMP-2的产生关系密切。侵袭性垂体腺瘤中基质降解活性程度高,TIMP-2 对IV 型胶原的降解起到一定抑制作用。结合第一部分,MMP-2、TIMP-2及 CD147 在基因与蛋白表达水平上具有一致性,CD147、MMP-2 及TIMP-2 可以作为垂体腺瘤侵袭性的有效指标。更多还原

【Abstract】 Part 1 The Relationship Between Protein Expression of MMP-2、 TIMP-2、CD147 and Invasiveness of Pituitary Adenomas Objective:To detect protein expression of MMP-2、TIMP-2 andCD147 in pituitary adenomas,and explore the relationship betweenexpression of MMP-2、TIMP-2 and CD147 and invasiveness of pituitaryadenomas and investigate its mechanisms and clinical value. Methods:In tissue samples from 55 pituitary adenomas,MMP-2、TIMP-2 and CD147 were detected by immunohistochemical staining.Therelationship between MMP-2、TIMP-2、CD147 and tumor invasivenesswere studied. Results: There were 32 invasive and 23 noninvasive cases in55 patients with pituitary adenomas. The intense and total expression rateof CD147 of pituitary adenomas patients with invasion were significantlyhigher than those without invasion (P<0.01),and the intense expression andtotal expression rate of MMP-2 of pituitary adenomas patients withinvasion were significantly higher than those without invasion (P<0.05),butthe intense expression and total expression rate of TIMP-2 in invasivepituitary adenomas were significantly lower than those withoutinvasion(P<0.01 or0.05).And the incidence of the invasiveness has nosignificantly difference with the sexuality of patients and secretion ofadenomas. Conclusion:Higher CD147、MMP-2 protein expression and lowerTIMP-2 protein expression may play important roles in the invasiveness ofpituitary adenoms and may be useful markers to predict theinvasiveness.But the invasiveness of Pituitary Adenomas has norelationship with the sexuality of patients and secretion of adenomas. Part 2 The Relationship Between mRNA Expression of MMP-2、 TIMP-2 、CD147 and Invasiveness of Pituitary Adenomas Objective:To detect messenger RNA (mRNA) expression of CD147、MMP-2、and TIMP-2 in pituitary adenomas,and explore the relationshipbetween expression of CD147、MMP-2、TIMP-2 mRNA and invasivenessof pituitary adenomas,and investigate its clinical value. Methods:CD147 、 MMP-2 and TIMP-2 mRNA expression wasdetermined in 32 invasive pituitary adenomas and 23 noninvasive pituitaryadenomas by reverse transcription polymerise chain reaction(RT-PCR). Therelationship between mRNA expression of CD147、MMP-2、TIMP-2 andtumor invasiveness were studied. Results: Both mRNA expression ofCD147 and MMP-2 increased in invasive pituitary adenomas (P<0.01).TIMP-2 mRNA was poorly expressed in invasive pituitaryadenomas (P <0.01) .There was a positive correlation between CD147 andMMP-2 at mRNA level,and there was a reverse correlation betweenMMP-2 and TIMP-2 at mRNA level. Conclusion:There were a high expression of CD147 and MMP-2 andlow expression of TIMP-2 in invasive pituitary adenomas, by whichcollagen IV inside basement membrance of pituitary tissue was damaged.Higher CD147、MMP-2 mRNA expression and lower TIMP-2 mRNAexpression may play important roles in the invasiveness of pituitaryadenoms. By compared with Part 1,the expressions of CD147、MMP-2 andTIMP-2 at protein level accord with that at gene level,so it was suggestedthat monitoring the expression of CD147、MMP-2 or TIMP-2 could be ofclinical value. MMP-2 and TIMP-2、CD147 are three useful tools forassessing the invasion of pituitary adenomas. CD147 has closelyrelationship with the creating of MMP-2 in the invasion of pituitaryadenomas.There have subtle relationship between MMP-2 and TIMP-2.更多还原