【作者】 孙巍；

【导师】 张静生；

【作者基本信息】 辽宁中医学院 ， 中西医结合临床， 2005， 硕士

【摘要】 目的:从美国加利福尼亚电鳐的电器官中提取纯化乙酰胆碱受体(AchR),主动免疫Lewis大鼠,复制实验性自身免疫性重症肌无力(EAMG)动物模型。方法:参照国内外文献,利用免疫亲和层析技术提取纯化AchR,采用SDS聚丙烯酰胺凝胶电泳测蛋白纯度,观察免疫后大鼠每日表现,并于初次免疫后第4周、第8周作肌电图,实验结束时取血清,检测乙酰胆碱受体抗体AchR-ab,最后处死大鼠,电镜观察神经肌肉接头。结果:模型组大鼠在初次免疫注射后的第17天开始相继出现肌无力症状,并有三只迅速恶化,一周内死亡。模型组其它发病大鼠经肌电图证实,衰退程度均大于15%,而佐剂组与正常组大鼠均无表现。模型组AchR-ab高于佐剂组与正常组值,P<0.05。电镜观察模型组神经肌肉接头处(NMJ)突触前后膜有改变。 结论:从美国加利福尼亚电鳐的电器官中提取纯化乙酰胆碱受体,主动免疫Lewis大鼠,成功复制出EAMG模型,为中药治疗重症肌无力的研究提供了理想的动物模型。更多还原

【Abstract】 Motive:Extracting pure AchR from American Torpedo California electric organ, immuning Lewis rats actively, duplicating EAMG animal model.Method:according to domestic and overseas literature, with immunoaffinity chromatography technology extracting pure AchR, adopting SDS polyacrylamide gel electrophoresis to detect purity of albumen, observing the immuned rats behaviour everyday, moreover making EMG in week 4 or 8 after the first immuning. When the experiment ended, the blood serum would be extracted to test AchR-ab. Finally executing the rats and observing neuromuscular junction with electron microscope.Result:The model group rats expressed myasthenia successively on the 17th day after the first immuning injection, besides there were 3 rats appearing worse and dying in a week. Affirmed by EMG, other rats of model group recession degree were exceeding 15% respectively.But neither did the adjuvant group and control group rats. The model group AchR-ab amount was higher than the adjuvant group and control group , p<0.05. Observing by electron microscope, we found that synapsis circa-membrane of the model group neuromuscular junction had changed.Conclusion: Extracting pure AchR from American Torpedo California electric organ, immuning Lewis rats actively, duplicating EAMG animal model successfully,Providing ideal animal model for the study of treating MG with Traditional Chinese medica.更多还原