【作者】 苗素云；

【导师】 李海峰；

【作者基本信息】 青岛大学 ， 神经病学， 2005， 硕士

【摘要】 目的 探讨协同刺激分子在实验性变态反应性神经炎发病中的作用。 方法 以18只2月龄的、体重1000克左右的雄性新西兰大白兔为研究对象,随机分为3组:FKG组背部多点皮下注射完全弗氏佐剂(FCA)、血蓝蛋白(KLH)和神经节苷脂(GM-1)混合制成的抗原乳剂;FK组注射完全弗氏佐剂和血蓝蛋白;FG组注射完全弗氏佐剂和神经节苷脂。各组动物均为6只,共注射3次。分别于注射前及第一次注射后每隔10天抽血提取淋巴细胞和血浆一次。采用逆转录多聚酶链反应技术(Reverse Transcription Polymerase Chain Reaction,RT-PCR)来半定量分析外周血淋巴细胞CD80和CD86 mRNA的表达水平;通过酶联免疫吸附实验(Enzyme-linked immunosorbent assay,ELISA)方法检测血浆抗GM1-IgG抗体滴度。 结果 FKG组、FK组CD80 mRNA的表达在免疫后10天开始增加,免疫后40天达高峰;免疫后各个时间点上FKG组、FK组CD80 mRNA的表达与FG组比较,差异有显著性(P<0.01),但FKG组与FK组比较差异无显著性(P>0.05)。FKG组、FK组CD86 mRNA的表达在免疫后10天开始增加,免疫后30天达高峰;免疫后各个时间点上FKG组、FK组CD86mRNA的表达与FG组比较,差异有显著性(P<0.01),但FKG组与FK组比较只有在免疫后40天差异有显著性(P<0.05),在其它时间点比较差异无显著性(P>0.05)。FKG组抗GM1-IgG抗体滴度在免疫后10天开始增加,免疫后40天达高峰;免疫后各个时间点上FKG组与FK组、FG组比较,差异有显著性(P<0.01),但FK组与FG组比较差异无显著性(P>0.05)。 结论 协同刺激分子CD80和CD86的表达通过载体蛋白活化T细胞,进而导致抗GM1-IgG抗体产生,在EAN的发病中起重要作用。更多还原

【Abstract】 Objective To investigate the roles of costimulatory molecules in experimental allergic neuritis(EAN).Methods EAN modles were established on 18 male New Zealand white rabbits. They were randomly divided into 3 groups. Subcutaneous injection took place on the rabbits of FKG with antigen emulsion that was mixed by FCA, KLH and GM1. FCA and KLH in FK group, FCA and GM1 in FG . There were 6 rabbits in each group and 3 injections on each rabbit. Lymphocyte and blood serum were collected before the first injection and every 10 days after the first injection. We analyse the expression of CD80 and CD86 mRNA in peripheral blood lymphocyte by Reverse Transcription Polymerase Chain Reaction(RT-PCR) and detect anti-GM1-IgG antibody titre in peripheral blood serum by Enzyme-linked immunosorbent assay (ELISA).Results The expression of CD80 mRNA in FKG and FK began to rise on the 10th day after immunity .It reached the peak on the 40th day after the injection.In FKG and FK it had a significant difference at each time point comparied with FG (P<0. 01),but there was no difference between FKG and FK(P>0.05). The expression of CD86 mRNA in FKG and FK began to rise on the 10th day after immunity .It reached the peak on the 30th day after the injection. In FKG and FK it had a significant difference at each time point comparied with FG (P<0. 01), between FKG and FK there was different only on the 40th day(P<0. 05),on the other time point there was no difference (P>0.05). Anti-GM1-IgG antibody titre in FKG began to rise on the 10th day after immunity. It reached the peak on the 40th day after the injection. In FKG it had a significant difference at each time point comparied with FK and FG (P<0. 01), but between FK and FG there was no difference (P>0.05).Conclusion Costimulatory molecules CD80 and CD86 play an important role in the activation of T cell mediated by protein carrier, then induced the production of anti-GM1 antibody, and then induced EAN..更多还原