【作者】 李涛；

【导师】 刘玉申； 张慧；

【作者基本信息】 青岛大学 ， 应用化学， 2004， 硕士

【摘要】 本课题探索出一种简易分光光度法测定万寿菊中叶黄素及浸膏中叶黄素的含量,与其他现有方法(HPLC,AOAC)相比,具有操作快速简便(单样检测时间大约缩短1/3—1/2)、溶剂耗量小、检测成本低、环境污染小(节省溶剂约1/2)、精确度高的特点,适用于万寿菊及其粗、精加工产品中叶黄素的检测。 本课题以万寿菊提取浸膏为原料,设计出一种分离纯化工艺,得到高纯度叶黄素。最后得到的产品经过红外、紫外、核磁氢谱、核磁碳谱确定为高纯度的叶黄素,并且通过HPLC定性和定量检测,产品的纯度达到80%以上,产率能够达到70%以上。且该叶黄素分离纯化工艺适于在工业生产中实现。 本课题还设计了叶黄素向玉米黄素转化的工艺,得到最佳转化反应条件,且得到较高的转化率(从原来的5%提高到近40%)。工艺简单,易于应用于生产,产业化推广。 本课题还在玉米黄素分子修饰制得生物效价极高的虾青素分子方面做了探索。从而增加了虾青素的一种新来源,而且这是一种由天然色素分子化合物经过分子修饰制取的来源,克服了国内外文献报道的几种来源中存在的过程复杂、成本高的缺点,适于工业化的实现,有很好的应用前景。更多还原

【Abstract】 The thesis mainly relates to a process to obtain xanthophylls concentrates of high purity from plant extracts. After drying under high vaccum, the crystalline is analyzed to be lutein with purity of more than 80 percent with IR, UV,NMR and HPLC.The thesis also relates to a process leading to the isomerization of Lutein to zeaxanthin and/or zeaxanthin-like epimers. The marigold extract is admixed with glycols and enough alkali. At the inert atmosphere, the mixture is heated to the temperature of about 100℃ for a period of time sufficient enough to complete the isomerization reaction. And the final substrate has a zeaxanthin content of about 40%, comparing to the initial content of 4.5%.A method to preparing .beta.-carotene derivatives such as canthaxanthin and astaxanthin is described. The method employs an in situ system to generate hypobromous acid as the oxidizing agent using a salt of sulfite, hydrogen sulfite orbisulfite in combination with a bromate salt. Astaxanthin and canthaxanthin are obtained in good yield with a significantly reduced reaction time.更多还原