【作者】 郭玉华；

【导师】 赵国琦；

【作者基本信息】 扬州大学 ， 动物营养与饲料科学， 2005， 硕士

【摘要】 第一部分 选择58头荷斯坦奶牛,采用3×3拉丁方设计分两阶段研究了泌乳期荷斯坦维生素A、D、E的需要量以及维生素A、D、E对乳脂、抗体、抗氧化性能及血清和牛奶中维生素A、D、E浓度等的影响,研究结果表明: 1.维生素A、D、E适宜添加量分别是:1250000IU/d、16000IU/d和1300mg/d。 2.随着维生素A、D、E剂量的增加,乳脂、乳蛋白、乳糖、总固体物质和游离脂肪酸的变化不显著（P>0.05）;各抗体浓度都有不同程度的增加,但上升不显著（P>0.05）;SOD和GSH有不同程度的增加,MDA有不同程度的下降,但变化不显著（P>0.05）;牛奶和血清中的矿物质变化不显著（P>0.05）;血红蛋白和淋巴细胞等有不同程度的上升,但变化不显著（P>0.05）;血清和牛奶中的维生素A和E极显著上升（P<0.01）。 第二部分 选用27头荷斯坦奶牛,采用L₉（3⁴）正交表的设计形式研究了维生素A、D、E之间的交互作用,研究结果表明: 1.维生素A、D、E在本实验的添加水平下,对所考察的奶产量、乳脂、抗体浓度等指标方面三者之间无交互作用（P>0.05）。 2.血清和牛奶中维生素A和E的浓度随着维生素A和E添加剂量的增加而极显著上升（P<0.01）。 第三部分 选择3头条件一致的荷斯坦奶牛,安装永久性的瘤胃瘘管、十二指肠瘘管和回肠瘘管;用ETDA-Co做标记物,采用了3×3拉丁方设计研究了不同维生素A、D、E添加水平对奶牛瘤胃、十二指肠、回肠和血液中维生素A、D、E浓度的影响、以及维生素A、D、E在小肠中的吸收情况,研究结果表明: 1.瘤胃、十二指肠、回肠和血液中维生素A、E浓度随着维生素A、E剂量的更多还原

【Abstract】 Part oneIn this experiment ,fifty eight lactating holsteins were chosed and 3 x 3 latin square design were adopted to research the requirement of vitanin A,D,E of lactating holsteins and the effect of different levels of vitanin A,D,E supplemented in the diets on milk fat, lactoprotein, milk suga, SCC,concentration of vitanin A,D,E in milk and serum and so on in two periods,The results showed that:l.The optimum level of dietary supplemental vitanin A,D,E of lactating holsteins is respectively 1250000IU/d, 16000IU/d and 1300mg/d .2.There is no interaction between VA and VE in statistics (P>0.05) ,but it is intensively suggested fom data that there is some interplay between VA and VE.3.There were significantly difference in milk fat, lactoprotein, milk suga, antibodies,SOD,MDA,GSH,NEFA,minerals (P>0.05) .4.As the concentration of vitanin A, E in diets increased,the oncentration of vitanin A,D,E in serum mounted up significantly (P<0.01) .Part twoIn this experiment ,twenty seven lactating holsteins were selected and crossing design were adopted to research the interaction of vitanin A,D,E and the effect of different levels of vitanin A,D,E supplemented in the diets on milk fat, lactoprotein, milk suga, SCC,concentration of vitanin A,D,E in milk and and serum and so on, The results indicated that:1.There is no interaction between VA and VE in statistics (P>0.05) .but it is intensively suggested fom data that there is some interplay between VA and VE.2. There were significantly difference in milk fat, lactoprotein, milk suga, antibodies,SOD,MDA,GSH,NEFA,minerals (P>0.05) .3. As the concentration of vitanin A, E in diets increased,the oncentration of vitanin A,D,E in serum mounted up significantly (P<0.01)Part threeIn this experiment ,three holsteins fitted with permanent rumen fistulate, proximal duodenum fistulate and ileum fistulate were used in 3x3 latin square design and ETDA-Co was used as marker to determine the effect of different levels of vitanin A,D,E supplemented in the diets on concentration of vitanin A,D,E in rumen, duodenum , ileum and serum and the asorption of vitanin A,D,E in small intestine,The results indicated that:1 .As the concentration of vitanin A, E in diets increased,the oncentration of vitanin A,D,E in rumen, duodenum , ileum and serum mounted up significantly (P<0.01) and the deposition of. vitanin A, E in small intestine also went up2. there were significantly difference in concentration of vitanin A, E at different sampling time (P<0.01) .更多还原