【作者】 刘新亚；

【导师】 马龙；

【作者基本信息】 新疆医科大学 ， 卫生毒理学， 2004， 硕士

【摘要】 目的:建立甜菜干粕中果胶的提取工艺,测定果胶多糖的含量,并进一步研究甜菜果胶对小鼠实验性高脂血症的影响。方法:首先用稀酸提取,乙醇沉淀法提取果胶,采用正交实验优选出最佳工艺。用果胶的含量测定代替传统的提取率为指标考察提取工艺。再用所提果胶进行调脂实验研究,采用雄性昆明种小鼠84只,按体重随机分为模型组、果胶高、中、低剂量组、齐墩果酸组(OA)及空白对照组,每组14只。模型组每日早晨灌喂一次高脂乳剂;果胶组和齐墩果酸组除每日早晨灌喂一次高脂乳剂外,下午灌喂受试物一次;空白对照组灌喂同容积的生理盐水。一周后,测定血清总胆固醇(TC)、甘油三脂(TG)和高密度脂蛋白胆固醇(HDL),并取一叶肝组织固定后病理检查。结果:筛选出的最佳提取条件为:20倍体积0.1M HCl,温度80℃,时间4小时;最佳醇沉条件为:提取液浓缩至密度为0.972,加4倍体积的95%乙醇,所得果胶含量最高。血清学检查发现中、高剂量果胶组都能降低TC,高剂量果胶组还能降低TG;病理学检查显示高剂量果胶组及OA组小鼠的肝脏脂肪沉积明显好于高脂模型组。结论:实验证实用酸提取、醇沉法可提取到含量较高的果胶。动物实验提示果胶可能具有降脂作用。更多还原

【Abstract】 Objectives:It was studied to extract pectin from dried beet pellet and effect of beet pectin on hyperlipemia mice .Methods: Acid extraction and ethanol sedimentary method were used to extract pectin from dried beet pellet. Orthogonal design was used to optimize the better acid extraction conditions. 84 health mice, male, were divided into six groups on the base of the weight , 14 mice each group. The experimental control group was administrated with high cholesterol,while the groups of Oleanolic acid(OA) and Pectin were administrated with their own sample except high cholesterol .One week later , the contents of serum total cholesterol (TC), triglycerides(TG) and HDL were measured ;the livers were fixation with formaline. Results:The optimum acid extraction from dried beet pellet was 20 times volumn 0. 1M HC1,80℃ for 4 hours. The optimum ethanol sedimentary conditions : density of the liquor was 0.972, the volumn of 95% ethanol was 4 times. Beet Pectin showed the actions of decreasing the levels of TC and TG. OA also decreased the levels of TC and TG. Conclusions: Pectin can be extracted by acid extraction and ethanol sedimentary method. Beet Pectin is an effective agent for hyperlipemia.更多还原