【作者】 杜娟；

【导师】 张微微；

【作者基本信息】 第三军医大学 ， 内科学， 2004， 硕士

【摘要】 目的 血小板衍生生长因子BB(PDGF-BB)在缺血再灌注中的功能及其与转化生长因子β1(TGF-β1)的表达关系。 方法 选取尸解人脑标本，分为脑梗死组及对照组；制备大鼠脑缺血再灌注(MCAO／R)模型，分为：a假手术组；b缺血再灌注组，缺血2h后依照再灌注时间的不同分为0h、6h、12h、24h、48h、3d及7d共6组；c组为MCAO2h／R24h尼莫通药物干预组；d组为TTC染色组测量缺血灶体积。a、b、c组各时相点取材，免疫组化显示脑PDGF-BB及TGF-β1的表达变化并进行定量分析。 结果 人脑标本：脑梗死组较对照组PDGF-BB和TGF-β1免疫阳性表达均增高。动物实验：缺血灶体积MCAO2h组最小，再灌注后增加，第3d时达到高峰，第7d时有所减小。药物干预组较无干预组缺血灶体积减小。PDGF-BB在对照组、MCAO2h组阳性表达无差异。随再灌注时间延长表达增加，7d后达高峰，药物干预组与无干预组相比阳性表达增多。TGF-β1在MCAO2h组较对照组阳性表达减低，再灌注6h后表达开始增多，48h达高峰，并持续至7d。药物干预组与无干预组相比阳性表达增加。 结论 脑缺血／再灌注损伤可以诱导PDGF-BB和TGF-β1表达，二者有自我保护、自我调节作用，促进缺血灶周围血管再生以及损伤修复；TGF-β1可能通过上调PDGF-BB起到神经保护作用；尼莫通可以增加这两个生长因子的表达，起到神经保护作用。更多还原

【Abstract】 Purpose The study aims at the function of platelet-derived growth factor BB (PDGF-BB) and the relation of PDGF-BB and TGF- ?1 in brain ischemia/reperfusion.Method Remove brain tissues from fatal cases with cerebral infarct and cases with non-cerebrovascular diseases. Rat model of ischemic reperfusion of cerebral middle artery was established. Rats were randomly divided into sham operation group, ischemia/reperfusion group, medicine treated group and TTC staining ischemia/reperfusion group. The ischemia/reperfusion group is divided into six subgroups according to reperfusion time difference such as Oh> 6h 12h. 24h. 3d and Id. MACO2h/R24h group was treated with Nimodipine. Using immunohistochemical staining method and color image analysis, the expression of PDGF-BB and TGF- ?1 in cerebral hippocampi were measured. The infarct volume was measured by TTC staining.Results Compared with control group, the expression of PDGF-BB and TGF- P 1 in infarct group in brain tissues from fatal cases increase. The ischemia volume (IV) in MCAO2h group is the smallest and the IV in MCAO2h/R3d group is the biggest. The IV in medicine treated group is smaller than the corresponding group without medicine. Expression of PDGF-BB in MCAO2h group remains the same as control group and increases after reperfusion with the peak value in 7d group. Expression of PDGF-BB in medicine treated group is higher than the expression in the group without medicine. Expression of TGF- P 1 decreases in MCAO2h group and increases after reperfusion with the climax in 48h until 7d. Expression of TGF- P 1 in medicine treated group is higher than the expression in the group without medicine.Conclusions Ischemia and reperfusion can induce expression of PDGF-BB and TGF-P 1. The two factors can protect and regulate themselves and promote blood vessels proliferation surrounding ischemic zone and help damage recovery. TGF- P 1 acts as a neuroprotection factor by the way of upregulation of PDGF-BB. Nimodipine can relieve cerebral tissue damage and increase expression of the two proteins and protect cells from damage.更多还原