【作者】 元超；

【导师】 周乐；

【作者基本信息】 西北农林科技大学 ， 植物学， 2004， 硕士

【摘要】 瑞香狼毒是瑞香科狼毒属植物，大毒，近年来，对其在防治农业病虫害及医药方面的应用报道很多，而对其在抗菌方面的报道却很少，本文针对其在抗菌方面的功效进行了系统的研究。我们先对瑞香狼毒根进行了系统的试管预试和薄层预试，初步确定出其中含有的主要化学成分：黄酮类、内酯香豆素类、挥发油、甾体萜类及少量有机酸和糖类。然后采用柱色谱分离纯化与生物测定跟踪相结合的研究方法来筛选活性成分。本实验选用了七种有代表性的菌种作示踪（列出七种），首先将实验材料用三种溶剂乙酸乙酯，乙醇，甲醇依次提取，根据抑菌试验情况决定对二乙部分进行再分离。二乙部分硅胶柱层析，共分得五部分：F1、F2、F3、F4、F5，同样方法, 进行生测，根据生测结果，决定对活性最强的F1、F2两部分再分离。F1与F2部分合并硅胶柱层析，共得五个组分：F1-1、F1-2、F1-3、F1-4、F1-5，同样方法，对五部分进行生测试验，由生测结果知：F1-4与F1-5抑菌活性最强。再用葡聚糖凝胶柱Sephadex LH－20对F1-4与F1-5两化合物进行纯化处理，通过采用两种差异较大的展开体系薄层检测，紫外灯与I2缸显色，均为单一斑点，证明化合物F1-4与F1-5均为纯品。通过对纯品F1-4与F1-5的物理、化学定性及有机波谱解析，结合文献资料，推断出化合物F1-4为新狼毒素B，化合物F1-5为狼毒色原酮；二者均为双黄酮类化合物, 且均为已知化合物。更多还原

【Abstract】 Stellera chamaejasme L. belongs to Stellera L. Thymelaeaceae, heavily toxic. In recent years, reports about its application on preventing agricultural pests, disease and medical science are enough, but the research on the anti-fungal is little. This thesis made a systematic research about its application on anti-fungal, on the basis of the predecessors’ work.The chemical constituents of stellera chamaejasme L.’s roots have been protested systematically by means of tube-test and thin layer chromatography (TLC). The main ingredients are flavanone, coumarins, steriod terpenoide and organic acid. We selected the most active compounds by column chromatography on silica, accompanied with antifungal tests.We have chosen seven kinds of fungal for trial(Bakeri Rehm、 Gibberalla zeae、 Alternaria solani、Fusarium bulbigenum、Exserohilum turcicum、Alternaria alternata、Phytophthora capsici Leon).The crushed roots of stellera chameajasmine L. were extracted with systemic solvent: ethyl acetate、ethanol、methanol. We decided to separate the ethyl acetate fraction according to the antifungal trial result. The ethyl acetate extract was separated by silica chromatography to afford five parts: F1, F2, F3, F4 and F5. An antifungal trial was made on the five parts by the same means. According to the results, we made a further separation on the significant active part: F1 and F2. The mixture of F1 and F2 was separated with silica chromatography to get another five parts: F1-1, F1-2, F1-3, F1-4 and F1-5. We made a third trial on the five parts and got two most antifungal active compounds in the end. We purified the two compounds through the column chromatography on Sephadex LH-20. They were speeded out with two big different solvent systems. They both were single point in the TLC, examined by ultraviolet lamp and I2 box. They were proved to be pure compounds.We can identified the two pure compounds F1-4 and F1-5 as neochamaejasme B and chamaechromone through physical、chemical quality analysis and organic spectrum determination, assisted with relevant papers. They both belong to biflavanone compounds and haven’t been reported for the first time.更多还原