【作者】 云蔓；

【导师】 南欣荣；

【作者基本信息】 山西医科大学 ， 口腔临床医学， 2004， 硕士

【摘要】 目的 评价骨多肽生长素(Bone Active Polypeptide，BAP)应用于小白鼠额面骨缺损区诱导骨再生修复的效果，探讨骨多肽生长素诱导成骨的机制。方法 健康昆明小白鼠72只，随机分3组，其中1组为实验组，2组为对照组，每组有24只小白鼠，在每只小白鼠额面骨建立骨缺损动物模型，用1％利多卡因额面浸润麻醉，切开皮肤、骨膜，显露额面骨，并制成3mm×2mm×1mm骨缺损区，止血完善。实验组植入骨多肽生长素复合珊瑚骨；对照A组植入单纯珊瑚骨；对照B组植入珊瑚骨复合地榆，分别用于修复额面骨缺损。严密缝合关闭手术创口，回笼饲养。术后7d、28d、90d分别处死24只小白鼠，每组8只，取材作切片。切取实验区额面骨4mm×3mm×1mm送检。在光镜下观察骨组织形态变化和新生骨结构，计量每视野新生成骨细胞数；在透射电镜下观察再生骨的超微结构变化。结果 组织病理学观察显示，术后7d，实验组与各对照组镜下表现差别不大，骨缺损区周围有破骨细胞、间充质细胞和大量炎性细胞浸润，骨变性坏死。术后28d，实验组可见炎性渗出明显吸收，成纤维细胞、毛细血管生长活跃，可见骨样组织和大量骨组织；对照A组可见炎性渗出吸收，骨缺损区周围有间充质细胞和成骨细胞；对照B组可见炎性渗出吸收，成纤维细胞、毛细血管生长活跃，骨缺损区周围有成骨细胞。术后90d，实验组可见新生骨组织范围扩大，钙化程度加强，几乎接近正常骨质结构；对照A组、B组皆可见骨缺损边缘有较多新骨沉积。于光镜下观察，计量3组新生成骨细胞数，运用单因素方差分析及两两比较，P＜0.01，实验组与各对照组有差异。再通过透射电镜观察得到进一步证明，术后90d，实验组骨缺损边缘有较多新骨沉积；对照A组、B组皆可见新骨样结构与骨创边缘的交界处仍有骨损线，分布有毛细血管及成骨细胞。结论 骨多肽生长素能诱导小白鼠额面骨再生，应用骨多肽生长素复合载体材料珊瑚骨可明显促进骨缺损的修复。骨多肽生长素(BAP)的诱导骨再生机制是含有多种与骨代谢有关的生长因子，从而诱导成骨细胞新生。更多还原

【Abstract】 Objective: To evaluate the effect on the frontal bone regeneration of the mice induced by Bone Active Polypeptide (BAP), to study the mechanism of bone regeneration induced by BAP. Methods: 72 Kun Ming mice were divided into 3 groups randomly, one was experimental group , the two were control groups, each was respectively 24. The mice were operated . Bone defects of 3 mm . 2 mm . 1mm in site were created on frontal bone with the use of local anesthesia, and then the defects had been filled with BAP composite Coralline in the experimental group; only Coralline in the control group A; Coralline composite Sanguisorba Officinalis L (SO) in the control group B. Postoperatively, the mice were killed at intervals of 7, 28 and 90 days, the specimens were examined histopathologically by light microscope and transmission electron microscope. Osteoblasts were counted and studied quantitatively by analysis of variance(ANOVA) to compare each group. Results: 7 days of postoperation, it was revealled that the bone defects were infiltrated by the white blood cells, degeneration and necrosis in all groups; after 28 days, the inflammation was absorbed, fibroblasts and new fine capillaries were developing actively, new bone formation were also found in the experimental group; the inflammation was absorbed in the control group A, and in the control group B, fibroblasts and many new capillaries were found. After 90 days, ossification was obvious in the experimental group, but not in the control groups. Osteoblasts were counted, P < 0.01, statistical analysis showed difference between the experimental and control groups. It was also proved by TEM. Conclusions: The frontal bone regeneration in the mice can be induced by BAP, and BAP composite Coralline was more remarkable. The mechanism of bone regeneration induced by BAP is that BAP is a variety of growth factors.更多还原