β-连环素和上皮钙黏附素免疫组化检测在大肠癌中的研究

【作者】 郝慧琴；

【导师】 卫建平；

【作者基本信息】 山西医科大学 ， 病理学， 2004， 硕士

【摘要】 目的：研究β-连环素(β-catenin，β-cat)和上皮钙黏附素(E-cadherin，E-cad)在大肠癌发生、发展中的作用和在预测肿瘤的转移潜能、评估预后及指导临床处理中有重要意义。方法：应用免疫组织化学染色方法ABC法检测20例正常人大肠黏膜、40例大肠腺瘤、20例腺瘤癌变及58例大肠癌组织的β-cat、E-cad的表达情况。大肠癌58例(结肠癌27例、直肠癌31例)，其中高分化腺癌15例，中分化腺癌24例，低分化腺癌19例；有淋巴结转移者33例，无淋巴结转移者25例；按Duke分期A期10例，B期12例，C期16例，D期20例；大肠腺瘤40例，大肠腺瘤癌变20例，20例正常人大肠黏膜取自经纤维结肠镜活检并经病理学诊断为非大肠肿瘤，且无大肠癌家族史就诊的患者作为正常对照。并对判别结果行统计学分析(x~2检验)。结果：正常大肠黏膜β-cat、E-cad呈胞膜阳性表达。大肠腺瘤、腺瘤癌变和大肠癌组织β-cat表达特征发生改变，胞膜表达缺失，呈胞质和／或胞核异位表达，腺瘤癌变β-cat异位表达率90％，显著高于大肠腺瘤(65％，P＜0.001)和大肠癌(65.5％，P＜0.05)；β-cat异位表达与大肠癌的组织分化程度、淋巴结转移、Duke分期等因素有密切关系。大肠癌β-cat、E-cad胞膜表达缺失率分别为77.6％、70.7％，显著高于大肠腺瘤(35％、30％)和腺瘤癌变(40％、35％)(P＜0.001)，且与大肠癌淋巴结转移和Duke分期有关。结论：β-cat异位表达可能参与了大肠癌发生、发展过程，是大肠癌发生的早期事件。β-cat、E-cad胞膜表达缺失可能与大肠癌的侵袭和转移有关。更多还原

【Abstract】 Objective To investigate the role of β -catenin and E-cadherin in the carcinogenesis and progression of colorectal carcinoma.Methods Expression of β -catenin and E-cadherin was examined immunohistochemically in 20 cases of normal colonic mucosa,40 cases of colorectal adenoma , 20 cases of colorectal adenoma carcinogenesis and 58 cases of colorectal carcinoma.Results In normal colonic mucosa, β -catenin and E-cadherin were detected in the cell membranes of epithelial cells, p-catenin expression was characterized by alterations in colorectal adenoma,adenoma canceration,and colorectal cancerous tissues;membranous expression fell into deletion,a cytoplasmic and/or nuclear ectopic expression occurred instead;thep-catenin ectopic expression rate of adenoma ( 65 % , P<0.01) and colorectal carcinoma(65.5%, P<0.05) ; P-catenin’s ectopic expression was related with the tissue differentiation degree of colorectal carcinoma,lymph node metastasis,and Dukes’staging.Deletion rates of membranous expression of p-catenin and E-cadherin in colorectal carcinoma were 77.6% and 70.7% respectively remarkably greater than those in colorectal adenoma(35.0%,30.0%) and in adenoma canceration(40.0%,35.0%)( P < 0.01) ,this also relating to colorectal carcinoma’s lymph node metastasis and Dukes’ staging.Conclusion The cytoplasmic and nuclearp-catenin expression(ectopic expression) might play a pivotal role in the carcinogenesis and progression of colorectal carcinoma . The delation of membranous p-catenin and E-cadherin expression might be related to the invasion and metastasis of colorectal carcinoma.更多还原