【作者】 陈一勇；

【导师】 杨桂姣；

【作者基本信息】 山西医科大学 ， 人体解剖学， 2004， 硕士

【摘要】 本实验采用SABC免疫组织化学染色方法，探讨了对照组、卵巢切除（OVX）组和卵巢切除+雌激素（OVX+E）组三组雌性Wistar大鼠海马结构内NOS阳性神经元数目、形态和分布的变化，并进行了计算机图像分析。结果显示：（1）海马结构各区均有NOS阳性神经元分布，细胞形态主要有锥体形和圆形两种。OVX组的CA1区与对照组相应区域的细胞相比，胞体排列较为紊乱，且突起变短；注射雌激素一个月的大鼠，海马结构CA1区细胞形态与对照组相似，细胞胞体排列整齐，突起较长。（2）OVX组大鼠海马结构CA1、CA3、CA4、下托区（S）和齿状回（DG）的NOS阳性神经元数量减少、面积比显著降低，灰度值明显增高，与对照组比较呈显著性差异；补充雌激素1月后，这三项指标均恢复，与对照组比较无显著性差异。结果提示：雌激素可能通过影响海马结构内NOS阳性神经元的表达来影响海马的功能。更多还原

【Abstract】 Effects Of Estrogen On NOS Positive-Neurons In Hippocampal Formation Of Female RatIn the present study ,we adopted the SABC immunohistochemical technique to observe the changes of morphology and distribution of NOS positive-neurons on hippocampus of three groups Wistar female rats (the reference ,ovariectomized and ovariectomized+estrogen group),which were analyzed with computer graphic analysis system. The study results show: (1) there are comprehensive NOS-positive staining cell groups in all regions of hippocampal formation. Pyramidal cell and oval cell are the two main cells in the three groups, but in CA1，cell dendrite is shorter in OVX group than in reference group. The effects can be reversed by estrogen given for 1 month. (2).The distribution of the neuron containing NOS in the hippocampal formation shows an obvious regional difference in the three groups .OVX in rats caused a decline in both number and area density of positive neurons in hippocampus CA1、CA3、CA4、S and dentate gyrus ，while the number and density of positive neurous in CA2 region have no remarkable changes.The effects can be reversed by estrogen given for 1 month. So it suggests that estrogen can stimulate the compose of nitric oxide, and estrogen may influence the learning and memory by affecting the expression of NOS in hippocampus .更多还原