【作者】 潘珊珊；

【导师】 郭书好； 徐石海；

【作者基本信息】 暨南大学 ， 分析化学， 2004， 硕士

【摘要】 海藻中含有丰富的次生代谢产物，且大多具有良好的生理活性。红藻是海藻的一个重要分支，含有大量的生理活性物质，对红藻的化学成分进行研究可以为寻找新的药物先导化合物提供重要的依据。 本论文首次对采自中国南海西沙群岛的红藻丝状乳节藻Galaxaura filamentosa的化学成分进行研究。通过硅胶柱层析，C-18反相柱层析，薄层层析及重结晶等方法，从中分离得到14个化合物，结合各种波谱手段鉴定了12个化合物的结构，这12个化合物都是首次从丝状乳节藻中分离得到，且其中1个为新化合物。 从10％乙酸乙酯-石油醚部分分离得到2个单体化合物，经IR，¹H NMR，EIMS分析，鉴定（Ⅰ）为棕榈酸，（Ⅱ）为硬脂酸。 从20％乙酸乙酯-石油醚部分分离得到化合物Ⅲ，经IR，¹H NMR，¹³C NMR分析确定为5a，8a-过氧麦角甾-6，22-二烯-3β-醇。 从50％乙酸乙酯-石油醚部分分离得到1个单体化合物（化合物Ⅳ），经过IR，¹H NMR，¹³C NMR，TOF-MS-ES+，TOF-MSMS ES+，HSQC，TOCSY，HMBC以及氨基酸组成分析，鉴定（Ⅳ）为环（N-取代亮氨酸-亮氨酸-N-取代亮氨酸-亮氨酸-亮氨酸），通过CA和Beilstein CrossFire检索，确证其为新化合物。初步抗肿瘤活性实验表明它对脑胶质瘤、肺腺癌和子宫内膜癌细胞有抑制作用，有关进一步活性实验正在进行中。 从60％乙酸乙酯-石油醚部分分离得到1个单体化合物（化合物Ⅴ），通过IR，¹H NMR，¹³C NMR，FAB-MS分析，确定（Ⅴ）为N-（2′-羟基二十四碳酰基）-1，3，4-三羟基-2-氨基-十八烷。 从10％乙酸乙酯-石油醚部分分离得到甾醇混合物A，经GC-MS分析，分离、鉴定出7个甾醇化合物（1-7）。 从18％甲醇-氯仿部分分离得到化合物Ⅵ，其结构有待进一步确证。更多还原

【Abstract】 Algae contain abundant secondary metabolites and many of them have good bioactivity. Red algae are an important branch of algae with plentiful bioactive compounds. Study of red algae’s component can provide a significant source of new drugs.In this dissertation, the constituents of red algae Galaxaura filamentosa collected from Xisha Islands were studied for the first time. Fourteen compounds were isolated by a combination of extraction and partitionation as well as multi-chro-matography. Twelve compounds’ structures of them were identified by spectra. All these compounds were firstly found from this algae and one of them was new compound.Four known compounds have been isolate from the EtoAc- petroleum ether portions. They are: palmitic acid; stearic acid; 5a,8a-epidioxyergosta-6,22-dien-3p-ol; N-(2-hydroxytetracosyl)-l,3,4-trihydroxy-2-amino-octadecan. Their structures were determined by IR, 1NMR, 13CNMR, EEMS, FAB-MS analysis.A new compound has been isolated from 50% EtoAc- petroleum ether portion. Its structure was elucidated as cyclo(-N-Me-Leu1-Leu1-N-Me-Leu2-Leu2-Leu3-) by IR, 1HNMR, 13CNMR, TOF-MS-ES+, TOF-MSMS ES+, HSQC, TOCSY, HMBC and amino acid analysis. During the primary bioassay, it exhibited inhibitory activity to tumor cell lines SWO-38, PC84045 and JEC. The ulterior research of is studying.From the 10% EtoAc- petroleum ether portion a mixture of sterol has been isolated. Based on GC-MS analysis, seven sterol compounds (1~7) were identified.Compound was isolated from the 12% methanol-chloroform portion. Its structure was determining.更多还原