【作者】 刘靖；

【导师】 洪文荣； 陈代杰；

【作者基本信息】 福州大学 ， 生物化学与分子生物学， 2004， 硕士

【摘要】 本研究以 4AD 为底物，筛选具有转化功能的微生物，得到了三个菌株：Beauveriabassiana HCB-00059 菌株、Rhizopus stolonife HCB-00643 菌株和 Rhizopus stolonifeHCB-00644 菌株。并从它们的转化产物中分离到 10 个转化化合物，其中 6 个结构已经确定。4 个分子量已经确定。1. 从 Beauveria bassiana HCB-00059 菌株的转化液中分离到化合物 BB059-1、 BB059-2、BB059-3 和 BB059-4，其中化合物 BB059-2 和 BB059-4 的化学结构已被确 定，分别是 6，11-二羟基雄甾烯二酮和 6-羟基睾酮。化合物 BB059-1 和 BB059-3 的分子量已被确定，均为 318。2. 从 Rhizopus stolonife HCB-00643 菌株的转化液中分离到化合物 RS643-1、RS643-2 和 RS643-3，其中化合物 RS643-2 和 RS643-3 的化学结构已被确定，分别是 6-羰基 睾酮和 6，15-二羟基雄甾烯二酮。化合物 RS643-1 的分子量已被确定，为 304。3. 从 Rhizopus stolonife HCB-00644 菌株的转化液中分离到化合物 RS644-1、RS644-2 和 RS644-3，其中化合物 RS644-2 和 RS644-3 的化学结构已被确定，分别为 6-羟 基雄甾烯二酮和14-羟基雄甾烯二酮。化合物RS644-1的分子量为已被确定，为318。4. 对影响三株菌株微生物转化过程的一些因素进行了考察，取得了较好的结果。更多还原

【Abstract】 The transformation of androstenedione (4AD) was studied to obtain valuable derivatives.A preliminary screening had showed that three strains were efficient for the production of4AD derivatives: Beauveria bassiana HCB-00059, Rhizopus stolonife HCB-00643, andRhizopus stolonife HCB-00644. The metabolites of three strains were isolated and purified.Ten pure compounds were obtained. The structures of six compounds had been identified,the molecular weights of the other four had been known. 1.Four compounds, BB059-1, BB059-2, BB059-3 and BB059-4 were isolated and purifiedfrom Beauveria bassiana HCB-00059 culture. The structures of BB059-2 and BB059-4 wereidentified as 6,11-dihydroxyandrostenedione, 6-hydroxytestosterone. The molecular weightsof the other two compounds , BB059-2 and BB059-4, were 318. 2.Three compounds, RS643-1, RS643-2 and RS643-3, were isolated and purified fromRhizopus stolonife HCB-00643 culture. The structures of RS643-2 and RS643-3 wereidentified as 6-oxo-testosterone and 6,15-dihydroxyandrostenedione. The molecular weightof RS643-1 was 304. 3. Three compounds, RS644-1, RS644-2 and RS644-3, were isolated and purified fromRhizopus stolonife HCB-00644 culture. The structures of RS644-2 and RS644-3 wereidentified as 6-Hydroxyandrostenedione and 14- hydroxyandrostenedione. The molecularweight of RS644-1 was 318. The parameters in the bioconversion process by three strains were optimized. The resultsshowed better progresses.更多还原