【作者】 刘远环；

【导师】 张兰；

【作者基本信息】 福州大学 ， 分析化学， 2004， 硕士

【摘要】 本论文研究了天然药物山药、石韦和五味子的毛细管电泳紫外检测或电化学检测分析方法及用CE对绿原酸的药物代谢动力学进行了初步研究。论文的第一部分介绍了毛细管电泳的简介和应用现状,重点评述了天然药物分析进展和毛细管电泳在天然药物中的应用，指出了本论文的研究课题。 论文第二部分采用毛细管区带电泳法成功了分离了山药中的尿囊素、胆碱和精氨酸。研究了电泳缓冲液的pH、电泳缓冲液的浓度、及外加分离电压等影响因素，在选定的最佳分离条件下，三组分在5分钟内达到基线分离。本方法具有操作简单、快速、方便及自动化程度高等特点，有望成为山药药材质量控制的常规检测方法。 论文第三部分采用胶束电动毛细管色谱法研究了天然植物五味子中的五味子甲素、五味子乙素和五味子酯甲的分离检测并成功的鉴别了北五味子与南五味子。讨论了电泳运行液、分离电压、进样时间及毛细管柱温对分离效果和检测灵敏度的影响。五味子甲素、五味子乙素和五味子酯甲的检测限分别为1.0(g/ml、0.9 (g/ml和0.2(g/ml。实验分别测定了北五味子和南五味子水提取样品和乙醇提取液样品，还成功的应用于模拟尿样品中五味子甲素、五味子乙素和五味子酯甲的含量测定。为市场上出售的北五味子与南五味子的鉴别提供了可靠、鉴别的方法。论文第四部分对天然药物石韦中绿原酸、槲皮素和山奈酚分离检测进行了研究，讨论了电泳缓冲液的pH、浓度、及表面活性剂因素对分离的影响。绿原酸、槲皮素和山奈酚的检测限分别为0.2×10-6μg/ml、0.4×10-6μg/ml、0.5×10-6μg/ml。用胶束电动毛细管色谱法成功的分离检测了不同地区石韦中绿原酸、槲皮素和山奈酚的含量。论文第五部分利用毛细管电泳法对绿原酸在兔血清中的药代动力学进行了初步研究，利用3P97计算软件计算出了药代动力学参数，根据这些参数，对被测定物在兔体内的代谢情况作了简单描述。 为了提高石韦中绿原酸、槲皮素的检测灵敏度，本文第六部分用毛细管电泳安培检测法研究了石韦中绿原酸和槲皮素的分离与检测。实验讨论了电泳缓冲液的pH、浓度、分离电压及进样时间对分离的影响。绿原酸和槲皮素的检测限分别是0.08 (g/ ml和0.02 (g/ml，比紫外检测低了将近一个数量级。本方法还成功的用于模拟牛血清样品中绿原酸和槲皮素含量的测定。更多还原

【Abstract】 Chinese herbal drugs are the gems of the five thousands years old civilization of our country. How to develop the advanced analytical methods for the research nature medicine is the challenge task in recent years. In this thesis, nature medicine- Dioscorea Opposita Thunb、Folium Pyrrosiae、 Fructus schisandrae was studied by capillary capillary chromatography with UV detection or electrochemical detection and Determination of pharmacokinetic parameter of chlorogetic acid has been carried out by Capillary electrophoresis.This paper consists of five chapters, in the first chapter of this thesis, the history of capillary chromatography was reviewed simply, the application on CE in recent years were introduced；the progress of the determination method for nature medicine was reviewed；the application of CE to nature medicine summarized emphatically.In the chapter II, High performance capillary electrophoresis (HPCE) was applied to the simultaneous separation and determination of allantoin, choline and arginine in Rhizoma Dioscoreae first time in this article. The optimal conditions of electrophoresis such as the pH and concentration of buffer solutions is studied. The three analytes could be well separated within five min by using a phosphate buffer and 24kV as the applied voltage. This method is a rapid, simple and useful technique for identification, separated and determination of allantoin, choline and arginine in Chinese traditional medicine- Rhizoma Dioscoreae. The method is also possible to be applicable to the quality control and directing the growing of Chinese medicine. In the chapter III, Micellar electrokinetic capillary chromatography with UV detection has been employed for the differentiation of North Fructus Schizandrae from South Fructus Schizandrae in the frist time. North Fructus Schizandrae contains a great of Schizandrin A、Schizandrin B and WuWeiZi ester A, but Schizandrin B are not prestent in South Fructus Schizandrae, so those crude drugs can be differentiated by determining their Schizandrin A、Schizandrin B and WuWeiZi ester A content. Effects <WP=4>of several important factors acidity and concentration of running buffer, separation voltage, injection time and detection potential were investigated to acquire the optimum conditions. The method was successfully applied to monitor these three compone nts in real samples suches as North Fructus Schizandrae plant fruit and South Fructus Schizandrae plant fruit distilled by water and ethanol.The method also applied to analyzed the simulated urine sample. In the chapter IV, a MEKC method was developed and applied to the simultaneous determination of kaempferol, quercetin and chlorogenic acid in Folium Pyrrosiae Under the optimum condition, the three analytes could be well separated completely within 8 min in a 70 cm capillary (75μm I.D.) of which 60cm was effective length for separation length with a separation voltage of 24kV in a 20 mmol l-1 of the borate buffer with 40 mmol l-1 SDS (PH=8.7). The detection limit of three components was 0.2×10-6μg/ml (chlorogenic acid)、0.4×10-6μg/ml (quercetin) and 0.5×10-6μg/ml (kaempferol)separately. Contents of chlorogenic acid, quercetin and kaempferol in the crude drug of Folium Pyrrosiae from different producing area could be easily determined by the proposed method with satisfactory results. In the chapter V，capillary electrophoresis was used for determination of chlorogenic acid in the blood serum, based on which some Pharmacokinetics parameters have been calculated. According to these parameters，the metabolization ofchlorogenic acid was described.In the chapterVI, in order to improve quercetin and chlorogenic acid sensitivity, in this thesis Capillary zone electrophoresis with electrochemical detection (ED) has been employed the separation and determination quercetin and chlorogenic acid in Rhizoma Dioscoreae. Effects of several important factors acidity and concentration of running buffer, separation voltage, injection time and detection potential were investigated to acquire the更多还原