【作者】 武荣兰；

【导师】 王吉德； 封顺；

【作者基本信息】 新疆大学 ， 物理化学， 2004， 硕士

【摘要】 本文以正辛醇-水体系模拟中药水煎液在人体内的分配情况，运用原子吸收光谱法、高效液相色谱法及紫可见分光光度法分别对三种常用中药当归、黄芪、黄芩中金属元素存在形态及可能的有效成分进行了分析。研究了不同靶位环境及不同配伍对中药中金属元素形态及基本有效成分的影响。第一章对中药中有效成分、微量元素、及药理的分析与研究方法的现状做了综述。第二章分析了中药当归、黄芪中7种金属元素在药材、水煎液中的含量、水煎溶出率及7种金属元素在模拟人体胃肠环境下的存在形态。测定了胃肠酸度下生物利用度高的醇溶态金属元素含量。并探讨了不同配伍对金属元素形态及溶出率的影响，分析结果表明，不同靶位环境及不同配伍对中药中金属元素形态影响很大。7种金属元素在小肠酸度下醇溶态含量均较高，应易于在小肠中被吸收，而不同比例配伍中以m（当归）: m（黄芪）=1:5时各金属元素溶出率最高。第三章用高效液相色谱法对不同靶位环境中的当归、黄芪及自制当归补血汤进行了指纹图谱分析。通过对指纹图谱的对比分析发现，中药当归在不同靶位环境下共有18个基本有效成分，而黄芪有22个基本有效成分。在不同的靶位酸度下，有效成分的数量及含量并不完全相同，说明在不同酸度下物质的结合及存在形态不同，在不同的靶位酸度下水煎液中的部分组分发生了解离或缔合，以更易被人体吸收的形态存在而起作用。第四章对中药黄芩中8种金属元素进行了形态分析。同时对不同靶位环境<WP=3>下黄芩进行了指纹图谱分析。结果发现，黄芩苷在不同靶位环境下含量差别较大，可能是黄芩苷与其他物质（如金属）结合或缔合并以结合形式存在。第五章考察了在不同体系中不同金属离子的存在对黄芩苷UV-Vis特征光谱的影响，以及金属离子对黄芩苷抗氧化活性的影响。发现黄芩苷在弱碱性条件下易与金属离子形成配合物；金属离子对黄芩苷的抗氧化性能有一定的增强作用，并探讨了其可能作用机理。更多还原

【Abstract】 In this paper, octanol-water system was adopted to study the distribution of decoction in stomach and intestine. Analyzed the metal speciation and effective chemical composition of three Chinese traditional medicine: Chinese Angelica root, Astragalus root，Scutellaria root. By FAAS,HPLC and UV-Vis spectrophotometer. The different acidities of digestive site, the different composition of medicine and compatibility of medicines have greatly affective the species of metal elements and the effective chemical composition.In chapter one, the study of Chinese Angelica root, Astragalus root and Scutellaria root were summarized imply.In chapter two, the total concentration, octanol– and water–solubility concentration of seven elements in medicinal materials or decoctions under gastric and intestinal acidity, and extract percent of TEs in Chinese Angelica root and Astragalus root, were determined, respectively, by FAAS, analyzed and compared. Results indicate that the different acidities of digestive site, different composition of medicine, and the compatibility of medicines, have greatly affected the existing species of metal elements, the pharmacological, activity of coordinated metal complex in decoctions. The octanol-solubility concentration of seven elements is higher under intestinal acidity. The extract percent of seven elements is higher when compatibility of ChineseAngelica root between Astragalus root is 1:5.In chapter three, HPLC was used to establish the fingerprints of Chinese Angelica root, Astragalus root and DanggBueTang under different acidities, the data were analyzed and compared. There are eighteen effective chemical compositions in Chinese Angelica root, twenty two in Astragalus root. The quantity and concentration of effective chemical compositions are different under different acidities. The different acidities of digestive sites, different compatibility of <WP=5>medicines have greatly affected the species of the effective chemical composition.In chapter four, the speciation of eight elements in Scutellaria root, were investigated, the fingerprint of Scutellaria root under different acidity were established. The concentration of Baicalin, main validity component in Scutellaria root, is different under different acidities; we presume that Baicalin could be combined with other components and exist in decoction or human body in combinative speciation.In chapter five, the UV-Vis spectrum characteristic and antioxidant action of Baicalin were studied when combined with different metal elements. The results indicated that Baicalin can coordinate with metal elements easily under low acidity. the antioxidant action of Baicalin was improved by metal element. The antioxidant mechanism of Baicalin with different metal elements in different systems were also investigated.更多还原