【作者】 祖英治；

【导师】 文景芝；

【作者基本信息】 东北农业大学 ， 植物病理学， 2004， 硕士

【摘要】 本论文以东北农业大学植物保护系植物源杀菌剂课题组研制的LS-1为主要研究药剂，以番茄叶霉病（Fulvia fulva）为防治对象，测定了LS-1对番茄叶霉病菌的毒力，LS-1对番茄叶霉病的田间防治效果，初步研究了LS-1对番茄的诱导抗病作用，同时进行了LS-1助剂的筛选，以及研究了在助剂筛选的基础上得到的助LS-1对番茄叶霉病的施药适期和室内接种防治效果。结果如下：田间防治试验中，LS-1（20.00g/L）对番茄叶霉病的防效达到62.46%，显著好于植物源药剂银果（0.334g/L）的防效50.19%、生物药剂菌克毒克（0.50g/L）的防效45.70%以及常用化学药剂甲基托布津（0.46g/L）的防效42.95%。在对诱导抗病性的研究中发现，番茄植株由LS-1诱导后，其叶片内苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和多酚氧化酶（PPO）活性均在第7d产生活性高峰，且明显高于对照。说明LS-1能够诱导番茄防御酶活性的显著增强，从而增强番茄抗叶霉病的能力。其中，LS-1的诱导使番茄体内的PAL活性比接种提前3d达到高峰，从而提前加强植株的苯丙烷类的代谢，达到预防番茄叶霉病的作用。在单一助剂的筛选中发现，LS-1（20.00g/L）加入助剂Y203（1.00g/L）对番茄叶霉病接种防治效果达77.89%，LS-1（20.00g/L）加入F302（0.50g/L）对番茄叶霉病的接种防治效果达到71.58%。在单一助剂筛选的基础上进行了LS-1与2种助剂混配的试验，初步确定LS-1原液与助剂的配方为LS-1：Y203：F302=200：30：3，其对番茄叶霉病的接种防治效果为79.36%。通过对助LS-1施药适期的研究，初步认定助LS-1防治番茄叶霉病的最佳施药时期为病菌侵入前7d至病菌侵入后7d，防效在54.09%~86.90%之间，施药间隔期为5d~7d。助LS-1对番茄叶霉病的室内接种防治试验中，40.00g/L和20.00g/L的助LS-1的防效分别为89.80%和86.35% ，显著优于常用化学药剂甲基托布津（0.46g/L）的防效68.14%、生物药剂菌克毒克（0.50g/L）的防效72.12%和植物源药剂银果（0.334g/L）的防效46.57%。更多还原

【Abstract】 The paper measured the toxicity of LS-1 to Fulvia fulva (Cookee ) Ciferri, and studied control effect of LS-1 on Tomato leaf mould in fields, and preliminarily studied induced resistance of tomato by LS-1, also screened adjuvants of LS-1, and studied the appropriate applying time of LS-1 and control effect of applying LS-1 mixted with adjuvants on Tomato leaf mould based on the experiment of screening adjuvant. The results were summarized as follows: The field control experiment showed that control effect of LS-1(20.00g/L) on Tomato leaf mould was 62.46%, and which was significantly better than the chemical fungicide (thiophanate-methyl),botanic fungicide(Yinguo) and Bio-fungicide(Junkeduke). Studies on induced resistance by LS-1 showed that PAL activity、POD activity and PPO activity in tomato leaves induced by LS-1 reached peaks in 7d at the same time, and significantly higher than Non-treatment. The results above indicated that LS-1 could induce activity of defensive enzyme in Tomato leaves , so as to amplify the capacity of tomatos’ resistance to Fulvia fulva (Cookee ) Ciferri.Especially, PAL activity in tomato leaves induced by LS-1 reached peak 3d ahead than inoculation only,so as to reach the purpose of precaution effect on Tomato leaf mould. The experiment of screenring singular adjuvant showed that control effect LS-1(20.00g/L)+Y203(1.00g/L) on Tomato leaf mould was 77.89%, and control effect of LS-1(20.00g/L)+F302(0.50g/L) on Tomato leaf mould was 71.58%. The mixture rate of LS-1 mixed with two kinds of adjuvants based on screening singular adjuvant was LS-1:Y203:F302=200:30:3, and its control effect on Tomato leaf mould was 79.36%. The appropriate time of applying LS-1 mixed with adjuvants was between 7d before fungus invading tomato and 7d after fungus invading tomato, control effect in this period was from 54.09% to 86.90%,and the time internal was 5d~7d. In the experiment of control effect on Tomato leaf mould indoors, control effect of LS-1(40.00g/L)and LS-1(20.00g/L) were 89.80% 86.35%,which significantly were higer than the chemical fungicide (thiophanate-methyl),botanic fungicide(Yinguo) and Bio-fungicide (Junkeduke). <WP=10>Graduate:Zu YingzhiMajor:plant pathologySupervisor:Prof. Wen Jingzhi更多还原