聚精丸对睾丸生精障碍性不育症的临床及实验研究

【作者】 卞廷松；

【导师】 徐福松；

【作者基本信息】 南京中医药大学 ， 中医外科， 2004， 硕士

【摘要】 本研究遵循中医理论，结合临床实际，从理论、临床两方面对睾丸生精障碍性不育进行系统深入的研究。在理论研究中对该病的中医历史渊源、病因病机、辨治方法等作了系统回顾，并对西医学关于本病的病因、病理生理、诊断方法及治疗进展进行综述。实验研究部分对生精障碍性不育患者的睾丸进行了常规病理切片检查和原位末端脱氧核苷酸转移酶杂交实验(Tdt-TUNEL法，terminal deoxynucleotidyl transferase，TDT，Terminal deoxynucleotidyl transferase-mediated deoxyuride triphosphate nick-end labeling，TUNEL)，初步发现了睾丸生精障碍性不育的凋亡规律。临床研究部分对本病患者的生精细胞凋亡指数与精子质量、精子倍体进行相关分析，并对导师徐福松教授的名方聚精丸的疗效及其对患者凋亡指数的影响进行临床观察，认为聚精丸治疗后的患者生精细胞凋亡指数接近正常，有药效学意义。更多还原

【Abstract】 Objective: To analyze the apoptotic regulation of vary types of spermatogenic cell, supporting cell and Ley dig cell in the testes of patients with infertility. To evaluate the effect of Chinese Traditional Medicine, the Pill of regenerated spermatozoa treatment, on the apoptotic change in the testes of infertilityMethods: In a prospective, nonrandomized, noncontrolled, clinical study, Testicle puncture biopsy were taken in 30 patients with dys-spermatid in the testes, and 5 cases of normal testes. Carry on the rule pathology check and the TUNEL (terminal deoxynucleotidyl transferase-mediated Dutp[deoxy-uridine triphosphate]nick end labeling) assay, (Td t ?TUNEL assay ), the changing of apoptotic indexes have been observed in vary types of spermatogenic celK supporting cell and Leydig cell in the convoluted seminiferous tubule of infertility .Result (s): Contrasted to normal group, apoptotic indexes obviously distinct in the convoluted seminiferous tubule of infertility, mainly expressing: the apoptotic indexes were increased in primary spermatocyte and secondary spermatocyte; the apoptotic indexes decreased in spermatid and sperm, the apoptotic indexes were decreased in primary spermatocyte and secondary spermatocyte while increased in ospermatid and sperm after treating with the Pill of regenerated spermatozoa in patients; and indexes of the semen parameters were obviously improved.Conclusion: From the apoptotic mechanism, we found that the apoptotic indexes were increased in primary spermatocyte and secondary spermatocyte while decreased in ospermatid and sperm in the testes of patients with infertility. The Pill of regenerated spermatozoa can readjusted the imbalance in spermatogenic cell with apoptotic turbulence, and obviously improved the spermatogenic function of the testes in infertilities.更多还原