【作者】 朱广慧；

【导师】 李家儒；

【作者基本信息】 武汉大学 ， 植物学， 2003， 硕士

【摘要】 薯蓣皂素（diosgenin）是从薯蓣属植物根状茎中分离出来的一种甾体化合物。它是合成甾体激素类药物的理想原料。它对人体及动物的生理作用已有广泛研究，但是它对植物生理作用并未引起特别的关注。油菜是我国重要的油料作物。本文研究了薯蓣皂素对油菜幼苗生长的影响及其生理生化基础，主要结果如下： 1 油菜种子经不同浓度的薯蓣皂素浸种处理后，0.5 mg／L的处理浓度显著促进油菜种子萌发。处理后的油菜种子萌发率提高，而且萌发的幼苗下胚轴伸长，干、鲜重增加。 2 用0.5mg／L的薯蓣皂素喷施处理二叶一心期油菜，0.5mg／L的处理浓度可以促进油菜幼苗生长。处理后的幼苗株高增加，叶片增大。 3 油菜种子经不同浓度的薯蓣皂素浸种处理后，0.5 mg／L的薯蓣皂素明显影响油菜幼苗生理代谢，子叶中可溶性蛋白质和可溶性总糖含量提高，过氧化物酶和过氧化氢酶的活性，Ca²⁺-ATP酶的活性上升。 4 二叶一心期油菜经0.5mg／L的薯蓣皂素喷施处理后，用ELISA测定内源激素含量，叶片生长素（IAA）和赤霉素（GA）含量提高。更多还原

【Abstract】 Diosgenin is steroid compound that is extracted from the tuber of Dioscorea L. It is a good material of synthesizing steroid medicines. Although the physiological effects of diosgenin on human and animals have been investigated widely, its physiological effects on plant have been ignored. Rape is an important oil crop in China. Here, the effects of diosgenin on seedling growth and its physio-biochemical basis in rape were investigated. The main results were as follows:1 Rape (Brassica napus L.) seeds were cultured in the solution containing diosgenin of different concentrations. The 0.5mg/L diosgenin increased prominently seed germination rates, the length of hypocotyls and the dry and fresh weight of leaf and stem.2 Rape seedlings were sprayed with 0.5mg/L diosgenin solution at thetwo-leaf and one-bud stage. The 0.5mg/L diosgenin promoted seedling growth. The seedling height and the leaf area increased after treatments.3 Rape seeds were cultured in the solution containing diosgenin of different concentrations. The different concentrations of diosgenin affected metabolism of rape seedlings, particularly at a concentration of 0.5mg/L. The treatments of 0.5mg/L diosgenin increased prominently the contents of soluble sugar, soluble protein and the activity of peroxidase, catalase, Ca2+ - ATPase in rape cotyledons.4 Rape seedlings were sprayed with 0.5mg/L diosgenin solution at the two-leaf and one-bud stage. ELISA (enzyme-linked immunosorbent assay) was used to determine the contents of endogenous hormones. The contents of endogenous IAA, GA increased comparing with the control.更多还原