【作者】 郭莹；

【导师】 赵景玮；

【作者基本信息】 福建农林大学 ， 农业昆虫与害虫防治， 2004， 硕士

【摘要】 小菜蛾Plutella lostella L．属鳞翅目菜蛾科，是世界性十字花科蔬菜最主要的害虫，也是抗药性发展最为严重的害虫之一。为了给小菜蛾的抗性监测提供理论依据，本论文研究了经历不同的饲养温度后，小菜蛾对毒死蜱的抗性以及相关的两种酯酶（乙酰胆碱酯酶、羧酸酯酶）活力变化情况。主要结果如下： （1）在10℃-30℃之内，随着饲养温度的上升，小菜蛾对毒死蜱的抗性以及酶活力也随之增加。 敏感品系对毒死蜱的LD₅₀与饲养温度的关系符合方程：y=-0.1144 x²+0.0803x-0.3649（R²=0.9526）； 抗性品系对毒死蜱的LD₅₀与饲养温度的关系符合方程：y=-0.0058x²+0.7025x+3.0554（R²=0.9281）； 敏感品系的乙酰胆碱酯酶比活力与饲养温度的关系符合方程：y=4E-06x²-7E-05x+0.0052（R²=0.9881）； 抗性品系的乙酰胆碱酯酶比活力与饲养温度的关系符合方程：y=-3E-06x²+0.0002x+0.0046（R²=0.9713）。 敏感品系的羧酸酯酶比活力与饲养温度的关系符合方程：y=0.006x²-0.0082x+0.438（R²=0.9674）； 抗性品系的羧酸酯酶比活力与饲养温度的关系符合方程：y=0.0031x²-0.0592x+0.8812（R²=0.9870）。 敏感品系的乙酰胆碱酯酶比活力与抗性的关系符合方程：y=0.0897x²-0.2487x+1.1598（R²=0.9634）；福建农林大学硕士学位论文小菜蛾对毒死蝉的抗性与饲养温度关系的研究一2- 抗性品系的乙酞胆碱醋酶比活力与抗性的关系符合方程:厂一0.2 996 xZ+o.955x+0.2675（RZ=0.9363）; 敏感品系的梭酸酷酶比活力与抗性的关系符合方程:厂0 .1 322::2一0.3 196x+1.1925（R2一0.9644）; 抗性品系的梭酸酷酶比活力与抗性的关系符合方程:产3 .1 38sx2一7.o93lx+5.1015（R，一0.9320）。 （2）5℃下饲养的小菜蛾幼虫对毒死蝉的敏感性低于常温20℃下饲养的小菜蛾。相应地，在5℃的低温条件饲养下小菜蛾的AchE和CarE比活力均大大高于在10℃一30℃范围内饲养的AchE和CarE比活力。 35℃的高温下饲养的小菜蛾的酶比活力受到抑制，相应地对毒死蟀的LDS。也有所降低。 （3）饲养于不同温度下的小菜蛾梭酸醋酶在受到毒死蝉的体内抑制之后，酶比活力的恢复速度不同，其中饲养于30℃的>20℃>10℃。更多还原

【Abstract】 Diamondback moth (Plutella xylostella L.), which belongs to Lepidoptera Plutellidae, is one of the most important worldwide pests, with high insecticide resistance, to cruciferous vegetables.is To provide theoretical basis for Plutella xylostella integrated management, studies to Plutella xylostella raised on different temperature on the resistance to chlorpyrifos , the change of AchE and CarE, were conducted. The results are summarized as follows:(1) Due to the rearing temperatures between 10 and 30, resistance and enzyme activity increase with the rising of rearing temperature. And, the regression equations are given below:a)for the relationship of LDso of susceptible strain and rearing temperature: y= -0.1144 x2+0.0803x-0.3649, R2=0.9760;b)for the relationship of LD50 of resistant strain and rearing temperature: y=-0.0058x2+0.7025x+3.0554, R2=0.9634;c)for the relationship of AchE activity of susceptible strain and raised temperature: y=4E-06x2-7E-05x+0.0052, R2=0.9940;d)foe the relationship of AchE activity of resistant strain and rearing temperature: y=-3E-06x2+0.0002x+0.0046, R2=0.9855;e)for the relationship of CarE activity of susceptible strain and rearing temperature: y=0.006x2-0.0082x+0.438, R2=0.9836;f)for the relationship of CarE activity of resistant strain and rearingtemperature: y=0.0031x2-0.0592x+0.8812, R2=0.9935;g)for the relationship of AchE activity of susceptible strain and resistance: y=0.0897x2-0.2487x+1.1598, R2=0.9815;h)for the relationship of AchE activity of resistant strain and resistance: y=-0.1996 x2+0.955x+0.2675, R2=0.9676;i)for the relationship of CarE activity of susceptible strain and resistance: y=0.1322x2-0.3196x+l.1925, R2=0.9820;j)for the relationship of CarE activity of resistant strain and resistance: y=3.1380x2-7.0931x+5.1018, R2=0.9654c(2) The sensitivity of chlorpyrifos on the larvae of diamondback moth raised on 5癈 is lower than that of raised on 20 C. Accordingly, the activities of AchE and CarE on the larvae of diamondback moth raised on 5 C are much more than that of raised within the range of 10C to 30 "C.The enzyme activity is inhibited of the diamondback moth raise on 35 C and with the reduction of LD50 to chlorpyrifos.(3) The recovery rate of the CarE activity is different after the inhibition of chlorpyrifos to CarE from the larvae of diamondback moth raised on different temperature.更多还原