【作者】 杨军方；

【导师】 李大力； 杨树林；

【作者基本信息】 南京理工大学 ， 生物化工， 2004， 硕士

【摘要】 从土壤中筛选得到一株产脂肪酶的野生细菌，命名为A6，根据菌体的形态显微观察和生理生化鉴定，初步鉴定该菌株为耶尔森氏菌属。 对其所产碱性脂肪酶进行分离纯化并进行相关酶学性质的研究，确定该酶反应的最适pH值为9.0，最适温度为40℃左右，分子量约为31kD，此酶是碱性脂肪酶。70℃处理10min酶活性丧失80％左右，40℃下保温60min酶活性还有80％左右；Ca²⁺、Mg²⁺等对酶活性有一定的激活作用，Zn²⁺、Fe²⁺、Mn²⁺和EDTA对酶活性有不同程度的抑制作用，而K⁺和Na⁺对酶活性基本没有影响。更多还原

【Abstract】 A strain of lipase producing bacterium was isolated form soil. It is identified as Yersinia van Loghem and named as A6.The optimum temperature of alkaline lipase is 40 ℃ and the optimum pH is 9.0. The molecular weight of the purified alkaline lipase is about 31kD. The optimum conditions of the bacterium fermentation for producing lipase and some properties of the enzyme are also investigated. This enzyme is classified as alkaline lipase. About 80% of the original activity is lost by heating at 70 ℃ for 10 minutes and 80% of the original activity is maintained by heating at 40℃ for 60 minutes.The enzyme activity is stimulated by Ca2+ and Mg2+, but is inhibited by Zn2+, Fe2+, Mn2+ and EDTA. K+ and Na+ have no influence on the enzyme activity.更多还原