【作者】 魏剑斌；

【导师】 付永胜；

【作者基本信息】 西南交通大学 ， 环境工程， 2004， 硕士

【摘要】 本文通过筛选脱色降解菌株的方法，对成都某印染厂的典型印染工艺中使范围较广的活性红PBL染料进行了脱色降解实验研究，结合实际工艺情况，筛选出了一株对活性红PBL有较高脱色降解活性的纯菌株TS4。考察了环境因子影响下该菌株对染料的脱色降解效果的影响，并采用常规细菌鉴定方法，依据该菌株的形态、生理生化特征，初步鉴定该菌株属芽孢杆菌属（Bacillus Sp.）。 细菌TS4可以在温度25～45℃、pH6～9的环境条件下保持较高生长率和脱色降解活性，其最佳脱色环境为37℃、pH=7.8、静置密封条件。氧作为环境因子对TS4菌脱色影响最大，静置密封条件下该细菌对染料的脱色率高要高于静置通气条件和摇床培养。此外酸碱度和温度对脱色也有影响。细菌TS4可以利用多种糖类、牛肉膏、酵母膏为碳源和能源，在对染料降解脱色的过程中可以不用附加氮源和Ca²⁺、Fe³⁺、Mg²⁺及微量元素等无机离子，最佳接种时期为该菌的对数生长期，适宜的接种量为10％，最大的染料耐受极限为200mg／L。同时，研究了TS4菌对金属离子的抗性，实验表明金属Hg²⁺、Ag²⁺离子对该菌有高毒性，对该菌的生长及脱色有抑制作用。随着染料浓度的增大，TS4菌对染料的脱色速率也逐渐降低。 此外，实验中分离出的纯菌株和混合菌株相比脱色降解性能较弱，说明混合菌种的对活性红PBL的脱色降解更具优势，这也说明该工艺系统的脱色降解微生物是一个综合协同作用的体系。更多还原

【Abstract】 The decolorization and degradation research of activated red PBL which is widely used during a typical technology in a printing and dyeing mill in Chengdu, combined the practical technology, by screening of efficient degrading bacteria is conducted and the influence of various environmental factors on decolorization and degradation efficiency is investigated. After cultivation of the microorganisms from several different samples, a activated red PBL degrading bacteria TS4 is founded by way of apprasing conventional bacterium, it is identified that the bacteria belongs to Bacillus Sp. , based on the strain’s form and the characteristic of its physiology .TS4 can keep the higher growth rate and the activeness of decolorization and degradation under the circumstance of the temperature form 25℃ to 45℃ and pH=6~9 , its best decolorization and degradation circumstance is the temperature of 37℃, pH=7.8 and being still sealed. The oxygen, as an environmental factor, is the most influencing factor about decoloraztion of TS4. The decoloraztion rate of it being still sealed is higher than it being still put in circulation of air and in shake cultivation. Moreover, acidity, alkalinity and temperature can affect the decolorization rate of TS4, This strain can use various kinds of saccharide, beef extraction and yeast extraction as carbon source and energy source . The additional nitrogen resources, Ca2+, Fe3+, Mg2+ , trace element and other inorganic ions can not be used during the process of degradation and decolorization of dye. The best inoculation period is its logarithmic growth period. The appropriate number of inoculation is 10%. The maximum of dye’s endurance is 200mg/L. TS4’s resistence to metal is also studied, the experiment indicates that the metal ion like Hg2+ and Ag2+ has the drastic toxicity to germ TS4 and it also suppresses germ TS4’s growth and decolorization. As dye’s concentration increased, the rate of germ TS4’s decolorization and degradation dye will decrease.Moreover, the characteristic of decolorization of the pure strain is weaker than the mixed strain. Both of them are separated form, that shows that the mixed strain has the advantage of decolorization and degradation of to the reactive red PBL. That also shows that the decolorization and degradation of the technical system of is a synthetical interactive system.更多还原