【作者】 闫志峰；

【导师】 李厚达；

【作者基本信息】 扬州大学 ， 动物学， 2004， 硕士

【摘要】 本文利用随机选择的位于Beagle犬不同染色体上的微卫星位点40个，从中筛选出多态性高、重复性好的16个微卫星位点，用PCR技术对三个Beagle犬种群的遗传多样性进行了分析和研究。旨在为Beagle犬遗传资源的合理利用及保护提供科学依据。 1，从已发表的40个微卫星位点中筛选出在本实验的三个Beagle犬种群中表现出多态性高、重复性好的16个微卫星位点，共检测到175个等位基因。 2，计算出16个微卫星位点的等位基因频率和特有等位基因频率，并以等位基因频率为基础，得出三个群体平均多态信息含量，群体基因杂合度，分别在0.55-0.85、0.66-0.88之间，从而说明各群体间保持了良好的多态性。 3，由实验结果得到了三个群体间的遗传距离D_A，结果表明三个群体间保持了遗传的一致性，并没有产生较大的基因变异。更多还原

【Abstract】 Sixteen microsatellite loci are selected from forty microsatellite loci on different chromosomes ,which shows highly polymorphisms and fine repeat. They are used in this experiment to analysis the genetic diversity of three Beagle canine by PCR analysis in order to offer basis of use and protection of Beagle canine genetics.1 The sixteen microsatellite loci which are selected from forty published ones show highly polymorphisms. We get 175 alleles.2 Calculating the allele frequency and the special allele frequency of sixteen microsatellites loci , and basing the allele frequency , we get the polymorphism information content and heterozygosity of three Beagle canine. The result are 0.55-0.85, 0.66-0.88 respecially. These results show that the genetic variation is abundant in every breed.3 In this experiment we get DA genetic distance. The results show that the genetic of three breeds keep consistency.更多还原