【作者】 李焕宇；

【导师】 邓虹珠；

【作者基本信息】 第一军医大学 ， 中药学， 2004， 硕士

【摘要】 目的：研究中药复方尿浊清(NZQ)对慢性肾小球肾炎(CGN)的治疗作用，并初步探讨其可能作用机制。方法：采用碳廓清和血清溶血素测定法，研究NZQ对小鼠非特异免疫功能和体液免疫功能的影响。通过观察NZQ对二甲苯致小鼠耳肿胀、对大鼠棉球肉芽肿的影响，研究NZQ的抗炎作用。复制两种CGN动物模型，即尾静脉注射阿霉素致大鼠微小病变肾病(MCN)模型和尾静脉注射阳离子化牛血清白蛋白(C-BSA)致膜性肾小球肾炎大鼠模型。连续给NZQ 4周或5周，分别观察NZQ对两种模型大鼠24 h尿蛋白量、肾功能、血清蛋白水平、脂质代谢产物、血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及肾脏组织病理学变化的影响。并观察NZQ对MCN大鼠模型血液流变学指标的影响，以及对C-BSA大鼠模型血清白介素-8(IL-8)、肿瘤坏死因子(TNF-α)的影响。结果：NZQ可提高小鼠的非特异性免疫功能、体液免疫功能；有显著的抗炎作用；可减少模型大鼠24 h蛋白尿量；降低血清肌酐、尿素氮水平；提高血清总蛋白、白蛋白水平；降低血清胆固醇、甘油三酯水平；提高SOD活性、降低MDA含量；降低血清IL-8、TNF-α水平；降低全血粘度、全血还原粘度；并可促进肾小球病理改变的修复。结论：NZQ对CGN有显著的治疗作用，对肾功能具有保护作用。NZQ可能通过调节机体免疫功能；改善肾小球肾炎时的血液高粘状态；清除氧自由基、抑制脂质过氧化反应；抑制IL-8、TNF-α等炎症因子的分泌，减轻炎症细胞在肾内的聚集，发挥其治疗作用。更多还原

【Abstract】 Objective: To study the therapeutic effect of Niaozhuoqing (NZQ) preparation on experimental rat models of chronic glomerulonephritis (CGN) and to explore the mechanism of the preparation. Methods: two kinds of animal models of CGN were reproduced, one was the minimal change nephropathy (MCN) rat model induced by tail-intravenous injection of Adriamycin and the other was membrane-damaged CGN rat model induced by tail-intravenous injection of cationized bovine serum albumin (C-BSA). After treated with NZQ preparation for 4 or 5 weeks, several measurements were done for 24-hour urinary protein contents, serum protein products of lipid metabolism, blood urea nitrogen (BUN), serum creatinine (SCr), activity superoxide dismutase (SOD) and malondialdehyde (MDA) in serum. In addition blood ratio viscosities were observed and the serum levels of interlenkin-8 (IL-8) and tumor necrosis factor (TNF- a ) were measured in rats with CGN. Also renal histopathological changes were observed by microscope. Results: NZQ given orally had obvious therapeutic effects on rats with CGN. In comparison with the untreated control group, the content of proteinuria in 24 hours, BUN, SCr, MDA, IL-8, TNF- a mean levels and the blood ratio viscosities in NZQ-treated groups were markedly lower (p<0.05, 0.01), serum SOD mean value was significantly higher (p<0.05) and the pathological changes of glomeruli were alleviated. Conclusion: NZQ had shown significant effect on the treatment of CGN by protection of renal function which might involve improvement of high coagulation state,elimination of oxygen free radicals, inhibition of lipid oxygenation, decrease the secretion of inflammatory cytokines including IL-8 and TNF-a , suppression the recruitment of inflammatory cells in the kidney.更多还原